Ultrasonography, Histopathological Udder Alterations And Bacteriological Investigations For Diagnosis Of Mastitic Goats | ||||
| Journal of Applied Veterinary Sciences | ||||
| Article 10, Volume 5, Issue 2, April 2020, Page 77-86 PDF (1002.34 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/javs.2020.85593 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ebtsam E.Z. Kotb  1; Fadel M.2; Ola A. Abd El-Fattah3; Ahmed Mohamed Azab4; Amal Z.A. Leil5
| ||||
| 1Animal Reproduction Research Institute, lHarm, Giza, Egypt. | ||||
| 2Diagnostic and Sonography Department, Animal Reproduction Research Institute, Giza, Egypt | ||||
| 3Giza Lab. Animal Health Research Institute Agricultural Research Center, Egypt. | ||||
| 4Department of Reproductive Pathology, Animal Reproduction Research Institute, Agricultural Research Center, Egypt | ||||
| 5Field investigation Department, Animal Reproduction Research Institute, Giza, Egypt. | ||||
| Abstract | ||||
| The purpose of this study was to evaluate the ultrasound and histopathological findings in comparison with the bacteriological examination to help in prognosis decision for udder alteration occurred by different cause and forms of mastitis. 40 Egyptian Baladi female goats (does) were examined for detection of mastitis during lactation period by clinical and laboratory examination include inspection, CMT, ultrasonography, pathological and bacteriology examination accompanied with antimicrobial susceptibility for isolated microorganisms (M.O.) for detection of udder alterations due to mastitis The results revealed the prevalence of subclinical and clinical mastitis was 25% and 12.5% respectively. 30% of the isolates werecoagulase-negative Staph. (CNS), while S. aureus and Strep. Spp < /em>. were 25% for each and E.coli represent 10% in subclinical milk samples, although these M.O. represented 40%, 30%,10% and 0% respectively in clinical milk samples. Ultrasonography of the parenchyma of healthy mammary gland appeared as a homogenous structure of average echogenicity filled with anechogenic content (milk). In contrast, the characteristic changes that occurred during all different mastitis phases in the mammary glandular parenchyma, teat and the milk appeared with different echogenicity. Histopathological features of tissue samples obtained by surgical biopsy technique described some different characteristic features of chronic, diffused interstitial mastitis lesions compatible with a longstanding subclinical infection. All forms of mastitis require microbiological confirmation for definitive diagnosis. Ultrasonography and histopathology give a clear prognosis for the status of the udder to minimize the impact of it. | ||||
| Keywords | ||||
| goat’s udder; histopathology; mastitis; microbiology; ultrasonography | ||||
| References | ||||
|
ABBA, Y.; ADAMU, L.; IGBOKWE, I.O.; HASSAN, S.U. AND SULE, D., 2014. Effect of clinical mastitis on the gross morphometry and histopathology of mammary glands of Sahel goats. Int. J. Livestock Res., 4, 1; 99-106
ABU, A. H., MHOMGA, L.I. AND AKOGWU, E. I. 2013. Assessment of udder characteristics of West African Dwarf (WAD) goat reared under different management systems in Makurdi, Benue State, Nigeria. African Journal of Agricultural Research, vol. 8, n. 25, p. 3255-3258.
ADAM, Z. E. A. S., RAGAB, G. A. N., AWAAD, A. S., TAWFIEK, M. G. AND MAKSOUD, M. K. M. A 2017. Gross anatomy and ultrasonography of the udder in goat J. Morphol. Sci., vol. 34, no. 3, p. 137-142
ADDIS MARIA FILIPPA, TIZIANA CUBEDDU, YLENIA PILICCHI, STEFANO ROCCA AND RENATA 2019. Chronic intramammary infection by Listeria monocytogenes in a clinically healthy goat – a case report. BMC Veterinary Research, 15:229
AHMED Y. F, OMIMA H. EZZO AND SAWSAN M. AHMED 2020. Some Udder Problems Associated with Productivity in Goats Egypt. J. Vet. Sci. Vol. 51, No.1, pp. 1-9
BANCROFT, J.D. AND GAMBLE, M. 2018. Theory and Practice of Histological Techniques. 8th Ed, Churchill Livingstone London, UK, and pp: 125-138 &328-329. BOURABAH, A., A. AYAD, L. BOUKRAA, S. M. HAMMOUDI, AND H. BENBAREK, 2013. "Prevalence and aetiology of subclinical mastitis in goats of the Tiaret Region, Algeria, ”Global Veterinaria, vol.11, no.5,pp.604–608, CLINICAL AND LABORATORY STANDARDS INSTITUTE in 2014. Performance standards for antimicrobial susceptibility testing; Twenty-Fourth Informational Supplement. Wayne, PA: Clinical and Laboratory Standards Institute. CLSI document; M100- S24
DANMALLAM FA AND PIMENOV NV 2019. Study on prevalence, clinical presentation, and associated bacterial pathogens of goat mastitis in Bauchi, Plateau, and Edo states, Nigeria, Veterinary World, 12(5): 638. EBTSAM E. Z. KOTB AND JEHAN A. GAFER, 2020. Molecular Detection Of Toxins And Disinfectant Resistance Genes Among Staphylococcus aureus Isolated From Dairy Cattle In Egypt.Journal of Applied Veterinary Sciences, 5 (1): 35-45 EBTSAM E. Z. KOTB, EL-SHAFAIE M.A. AND SAMEH A. IBRAHEM 2018.Molecular characterization of toxigenic and antibiotic-resistant of S.aureus of recurrent bovine mastitis. Assuit Vet. Med. J. Vol.64 No.158, pp. 1-8 EBTSAM E.Z. KOTB, ASHRAF M. ABU-SEIDA AND MOSTAFA S. FADEL 2014a. The Correlation Between Ultrasonographic and Laboratory Findings of Mastitis in Buffaloes (Bubalus Bubalis). Global Veterinaria 13 (1): 68-74
EBTSAM E. Z. KOTB, RAGHIB R. W. AND OLA A. ABDEL FATTAH 2014b. The economic impact of mastitis in some dairy farms in Egypt. J. Egypt Vet. Med. Assoc. 74,no 4, 579-595
EBTSAM E.Z. KOTB 2008. Some studies on the immune response of lactating bovine mammary gland to different mastitic vaccines. Thesis of Ph degree in Vet. Med. Sc. Cairo university
EBTSAM E.Z. KOTB 2001.Detection of bacterial antigens in milk s from clinical cases of bovine mastitis. Thesis of master degree in Vet. Med. Sc. Cairo University
EL-SAYED, E.H.; EL-SHAFIE, M.H.; SAIFELNASR, E.O.H.; ABU EL-ELLA, A.A. 2009. Histological and histochemical study on mammary gland of Damascus goats through stages of lactation. Small Ruminant Research, 85;11-17. EL-SAYED, E.H.; HASSAN, L.R.; SAIFELNASR, E.O.H.; ASHMAWY, T.A.M. 2013. Ultra-structure study of the mammary gland in Zaraibi goats during different stages of lactation. Egypt J Sheep Goat Sci., 8;33-45.
FASULKOV IVAN , MANOL KARADAEVA, NASKO VASILEVA, RADOSTIN SIMEONOVB, VALENTINA URUMOVAC , AND ELEONORA MLADENOVA 2015: Ultrasound and histopathological investigations of experimentally induced Staphylococcus aureus mastitis in goats. Small Ruminant Research 129 ;114–120.
FASULKOV M.; KARADAEV AND M. DJABIROVA 2014. Ultrasound measurements of teat structures in goats. Revue Méd. Vét., 165, 5-6, 188-192
FASULKOV, IR., YOTOV, S., ATANASOV, A. AND ANTONOV, AL. 2013. Evaluation of different techniques of teat ultrasonography in goats. Journal of the Faculty of Veterinary Medicine, Istanbul University, , vol. 39, n. 1, p. 33-39
FASULKOV, IR., GEORGIEV, PI., ANTONOV, AL. AND ATANASOV, AS 2010. B-mode ultrasonography of mammary glands in goats during the lactation period. Bulgarian Journal of Veterinary Medicine, vol. 13, p. 245-251.
GABLI Z., DJERROU Z., GABLI A.E. AND BENSALEM M. 2019. Prevalence of mastitis in dairy goat farms in Eastern Algeria , Veterinary World, 12 (10):1563- 1572
GUILHERME PUGLIESI, GABRIELA DALMASO DE MELO, GILMER ARANTES ATAÍDE JR, CARLOS AUGUSTO GONTIJO PELLEGRINO, JÚLIO BARBOZA SILVA, CECÍLIA CONSTANTINO ROCHA, IGOR GARCIA MOTTA, JOSÉ LUIZ MORAES VASCONCELOS, MARIO BINELLI 2018. Use of Doppler ultrasonography in embryo transfer programs: feasibility and field results. Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianopólis, SC, Brazil, August 16th to 18th
HANAA ASFOUR, A.E.; ABEER ANWER, M.; NABEEH, A.M. AND ABEER EL-METWALLY, E. 2016. Some studies on Pasteurella multocida as a causative agent of mastitis in dairy cows and ewes. Assiut Vet. Med. J., 62, 150; 143-156. HUSSAIN, R.; KHAN, A.; JAVED, M.T. AND RIZVI, F. 2012. Possible risk factors associated with mastitis in indigenous cattle in Punjab, Pakistan. Pak Vet J, 32, 4; 605-608. IBRAHIM, H.H.; JESSE, F.F.A.; ABBA, Y.; CHUNG, E.L.T.; MARZAI, A.D.; HARON, A.W.; ZAMR-SAAD, M.; OMAR, A.R. AND SAHAREE, A.A. .2016: Clinical and histopathological study on reproductive lesions caused by Pasteurella multocida type B2 immunogens in buffalo heifers. Bulgarian J. Vet. Med. JAI W, LEE S.N., OBRIEN A., JUIDRY J., MAX K.A. AND SHAFAR W.A. 2005. Effect of the trivalent vaccine against Staph. aureus mastitis lymphocytes
subpopulation antibody production and neutrophil phagocytosis. Canadian J. Vet. Res., 69: 11-18.
MAHLANGU PRECIOUS, NAOMI MAINA AND JOHN KAGIRA 2018. Prevalence, Risk Factors, and Antibiogram of Bacteria Isolated from Milk of Goats with Subclinical Mastitis in Thika East Subcounty, Kenya J. of Vet. Med. Volume 2018, Article ID 3801479, 8 pages
MBINDYO C. M. AND BEBORA L. 2014. A cross-sectional on the prevalence of subclinical mastitis and antimicrobial susceptibility patterns of the bacterial isolates in milk samples of smallholder dairy goats in Kenya," American Journal of Research Communication, vol.2,no.8,
NATIONAL MASTITIS COUNCIL (NMC) 2011.3rd. International Symposium on Mastitis and Milk Quality Proceedings; 2011: 38-42
NOGUEIRA FILHO, A., FIGUEIREDO JÚNIOR, C.A. AND YAMAMOTO, A. 2010. Mercado de Carne, Leite e Pele de Caprinos e Ovinos no Nordeste. Série Doc. Etene, Fortaleza, p.125
PRIYA, S. AND S. AYODHA, 2016. “Bacterial and antibiogram studies of milk samples of clinical mastitis in goats,” IOSR Journal of Agriculture and Veterinary Science, vol.9, no.6, pp. 33–35. QUINN, P.J., MARKEY, B.K., CARTER, M.E., DONELLY, W.J. AND LEONARD, F.C. 2002. The bacterial cause of bovine mastitis. In: Veterinary Microbiology and Microbial Diseases. Blackwell Science Ltd, a Blackwell Publishing Company, Oxford, UK. p465-75.
SANTOS V.J.C., K.M.M.G. SIMPLÍCIO, D.C.C. SANCHEZ, V.T. ALMEIDA, P.P.M. TEIXEIRA, L.N. COUTINHO, L.F.S. RODRIGUES, M.E.F. OLIVEIRA, M.A.R. FELICIANO AND W.R.R. VICENTE 2014. Conventional and Doppler ultrasonography on a goat with gangrenous mastitis. Arq. Bras. Med. Vet. Zootec., v.66, n.6, p.1931-1935, 2014
SHAHEEN, M.; TANTARY, H.A. AND NABI, S.U. 2016. A Treatise on bovine mastitis: Disease and disease economics, etiological basis, risk factors, impact on human health, therapeutic management, prevention and control strategy. J. Adv. Dairy Res., 4, 1; 10 pages. SOUSA, F.C.; PAULA, A.R.; CAMPELO, L.S.; MELO. L.M.; FREITAS, V.F. AND TEIXEIRA, D.A. 2017. Mammary biopsy in induced lactating goats: Assessment of post-biopsy milk properties and ultrasonographic appearance. Ciencia Animal Brasileira Goiânia, 18, 1-7.
SZENCZIOVA I. AND STRAPAK P. 2012. Ultrasonography of the udder and teat in cattle, perspective measuring technique. Slovak J. Anim. Sci., 45 (3): 96-104. | ||||
|
Statistics Article View: 649 PDF Download: 719 |
||||
