Diterpenoids Profile of E. paralias and E. geniculata Using UPLC-ESI/MS Spectrometry
Ghani, A., eltomy, S., eldougdoug, W., hassan, H., Badr, W. (2020). Diterpenoids Profile of E. paralias and E. geniculata Using UPLC-ESI/MS Spectrometry. EKB Journal Management System, 63(12), 5039-5053. doi: 10.21608/ejchem.2020.25113.2486
Afaf Said Ghani; sayed abdelhamid eltomy; Wagdi Ibrahim eldougdoug; hanaa mohamed hassan; Wafaa Hassan Badr. "Diterpenoids Profile of E. paralias and E. geniculata Using UPLC-ESI/MS Spectrometry". EKB Journal Management System, 63, 12, 2020, 5039-5053. doi: 10.21608/ejchem.2020.25113.2486
Ghani, A., eltomy, S., eldougdoug, W., hassan, H., Badr, W. (2020). 'Diterpenoids Profile of E. paralias and E. geniculata Using UPLC-ESI/MS Spectrometry', EKB Journal Management System, 63(12), pp. 5039-5053. doi: 10.21608/ejchem.2020.25113.2486
Ghani, A., eltomy, S., eldougdoug, W., hassan, H., Badr, W. Diterpenoids Profile of E. paralias and E. geniculata Using UPLC-ESI/MS Spectrometry. EKB Journal Management System, 2020; 63(12): 5039-5053. doi: 10.21608/ejchem.2020.25113.2486

Diterpenoids Profile of E. paralias and E. geniculata Using UPLC-ESI/MS Spectrometry

Egyptian Journal of Chemistry
Article 33, Volume 63, Issue 12, December 2020, Page 5039-5053  XML PDF (1 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2020.25113.2486
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Authors
Afaf Said Ghani1; sayed abdelhamid eltomyorcid 2; Wagdi Ibrahim eldougdoug3; hanaa mohamed hassan email 4; Wafaa Hassan Badr5
1department of Pharmacognosy, Pharmacy , Zagazig University.
2National Research centre Tanning lab.
3Department of Organic Chemistry, Faculty of Science, Banha University
4pharmacognosy department, zagazig university.
5Department of Pharmacognosy Faculty of Pharmacy, Zagazig University
Abstract
Euphorbia species contain series of macrocyclic diterpenoids which present as mono, di and sometimes triesters and were reported to possess several and diverse biological activities. The aim herein was to use the positive ion mode ultraperformance electron spray ionization mass spectroscopy (UPLC-ESI-MS) as an analytical tool for rapid screening and tentative identification of diterpenoids in the methylene chloride fractions of Euphorbia paralias and Euphorbia geniculate. The identification of compounds was based on ESI-MS and ESI-MS/MS fragmentation pattern, in addition to comparison with reported literature. Five classes were determined including tigliane, ingenane, lathyrane, daphnane and jatrophane diterpenoids esters. Each class of the detected diterpenoids obtained its characteristic ESI-MS/MS fragmentation which facilitates its identification. As a result, a total of fifty two diterpenoids were identified and characterized in E. paralias and E. geniculate. All of these compounds are first report in the two plants by this method. The results demonstrated that the used method could be a rapid, effective analytical tool for screening and characterization of diterpenoids in the complex systems of plants extracts.
Keywords
Keywords: Euphorbia; Terpenoids; UPLC-ESI-MS; ingenanes; and tiglianes
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