RECOVERY RATE AND QUALITY OF EMBRYOS AS AFFECTED BY REPEATED SURGICAL COLLECTION FROM NEW ZEALAND WHITE AND BALADI RABBIT DOES. | ||||
| Journal of Animal and Poultry Production | ||||
| Article 3, Volume 1, Issue 5, May 2010, Page 175-186 PDF (444.9 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jappmu.2010.86200 | ||||
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| Authors | ||||
A. E. Abdel-Khalek 1; Sh. M. Shamiah2; Rania R. Ali1
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| 1Anim. Prod. Dept., Fac. Agric., Mansoura University. | ||||
| 2Anim. Prod. Res. Instit., Agric. Res. Center. | ||||
| Abstract | ||||
| Total of 12 New Zealand white (NZW, n=6) and Baladi black (BB, n=6) rabbit does (6-7 mo of age and 3.25-3.50 kg LBW) as embryo donors and two fertile bucks one from each breed (7.5-8 mo of age and 3.5-4 kg LBW) for natural mating during the experimental period were used in this study were used to study the effect of repeated surgical collection on quality and recovery rate of embryos recovered from does in each breed induced to ovulate by GnRH at insemination. Ovulation was induced by an i.m. injection of 20 mg GnRH and embryos were surgically collected 72–76 h post-mating by ventral midline laparotomy. After each of repeated embryo collection for 3 times, the number of corpora lutea (CLs), normal (NF) and hemorrhagic (HF) follicles on the ovary and the number of normal (NE) and unviable (UVE) embryos recovered were recorded. Then ovulation rate (OR) and embryo recovery rate (ERR) were calculated. Results show that number/doe of NF (23.8 vs. 16.6), HF (6.6 vs. 4.2) and total follicles (TF, 30.4 vs. 20.8) was higher (P<0.05) and ovulation rate (OR) was lower (28.3 vs. 42.8%, P<0.05) in Baladi than in MZW does. Number of CLs was 8.9 and 8.6/doe in NZW and Baladi rabbits, respectively. Average number/doe of NF, HF and TF was not affected by collection number. Average number of CLs/doe and OR increased (P<0.05) by increasing collection number, being 7.6 and 27.1%, 8.8 and 35.6% and 10.0 and 41.3% with the 1st, 2nd and 3rd collection, respectively. Average number of UVE/doe (1.9 vs. 1.3), TE (7.5 vs. 5.7) and ERR (87.0 vs. 64.0%) was higher (P<0.05) in Baladi than in MZW does. The difference in number of NE/doe was insignificant between NZW (4.4) and Baladi does (5.6). Average number of NE and TE/doe and ERR was higher (P<0.05) with the 1st (6.2, 7.3 and 96.1%) and 2nd (6.8, 8.3 and 94.3%) collections than with the 3rd collection (1.9, 3.7 and 37%). Average number of UVE/doe was 1.1, 1.5 and 1.8 at three successive collections. Such results may reflect the save side of embryo surgical recovery for two collections on number of embryos/doe and embryo recovery rate as a results of increasing number of UVE and ERR by increasing collection number up to three times. | ||||
| Keywords | ||||
| Rabbit does; surgical collection; follicles; embryos; recovery rate | ||||
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