Silver nanoparticles: A potential antibacterial and antibiofilm agent against biofilm forming multidrug resistant bacteria
El-Shennawy, G., Abd Ellatif, R., Badran, S., El-Sokkary, R. (2020). Silver nanoparticles: A potential antibacterial and antibiofilm agent against biofilm forming multidrug resistant bacteria. EKB Journal Management System, 1(2), 77-85. doi: 10.21608/mid.2020.28087.1008
Gehan El-Shennawy; Randa Abd Ellatif; Shahenda Badran; Rehab El-Sokkary. "Silver nanoparticles: A potential antibacterial and antibiofilm agent against biofilm forming multidrug resistant bacteria". EKB Journal Management System, 1, 2, 2020, 77-85. doi: 10.21608/mid.2020.28087.1008
El-Shennawy, G., Abd Ellatif, R., Badran, S., El-Sokkary, R. (2020). 'Silver nanoparticles: A potential antibacterial and antibiofilm agent against biofilm forming multidrug resistant bacteria', EKB Journal Management System, 1(2), pp. 77-85. doi: 10.21608/mid.2020.28087.1008
El-Shennawy, G., Abd Ellatif, R., Badran, S., El-Sokkary, R. Silver nanoparticles: A potential antibacterial and antibiofilm agent against biofilm forming multidrug resistant bacteria. EKB Journal Management System, 2020; 1(2): 77-85. doi: 10.21608/mid.2020.28087.1008

Silver nanoparticles: A potential antibacterial and antibiofilm agent against biofilm forming multidrug resistant bacteria

Microbes and Infectious Diseases
Article 8, Volume 1, Issue 2, August 2020, Page 77-85  XML PDF (495.82 K)
Document Type: Original Article
DOI: 10.21608/mid.2020.28087.1008
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Authors
Gehan El-Shennawy1; Randa Abd Ellatif1; Shahenda Badran email 1; Rehab El-Sokkaryorcid 2
1Medical Microbiology and Immunology department, Faculty of medicine, Zagazig University, Egypt
2Associate professor of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Egypt
Abstract
Background: Multi-drug resistant (MDR) bacteria are seriously endangering the antibiotics. Different alternative strategies are needed to reinforce antibiotics, of, these; nanostructured materials may play a fruitful role. This study aimed to investigate the antibacterial and antibiofilm activity of silver nanoparticles (AgNPs) against MDR bacteria. Methods: In a cross-sectional study, a total of 33 methicillin resistant Staphylococcus aureus (MRSA) and 52 MDR Pseudomonas aeruginosa (P. aeruginosa) isolates were recovered from intensive care units’ (ICUs) admitted patients over a period of 9 months, from December 2017 to August 2018. The antibacterial activity of AgNPs on the clinical isolates of MRSA and MDR P. aeruginosa was assessed by minimum inhibitory concentrations (MICs) using broth microdilution method. The minimum bactericidal concentrations (MBCs) were determined as the lowest concentrations required to kill 99.9% of the initial inoculum. Tissue culture plate method was used to evaluate the antibiofilm activity. Results: The MIC and MBC values ranged from 1 to 16 μg/ml and 2 to 64 μg/ml, respectively. Silver nanoparticles exerted a significant antibiofilm activity against MRSA and MDR P.aeruginosa at all tested concentrations, recording a maximum inhibition value of (82%) and (91%), respectively. Conclusions: AgNPs exhibited a considerable antibacterial and antibiofilm, effect; it could represent a promising weapon in the fight against biofilm forming MDR organisms.
Keywords
silver nanoparticles; multidrug resistant; MRSA; P. aeruginosa
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