ENHANCED SILYMARIN ACCUMULATION AS INFLUENCE OF MEDIUM COMPOSITION IN CELL SUSPENSION CULTURES OF Silybum marianum (L.) GAERTN.

Rabie, Kawthar A. E.; Abd El-Aal, Mona S.; Manaf, H. H.

doi:10.21608/jpp.2010.86356

	ENHANCED SILYMARIN ACCUMULATION AS INFLUENCE OF MEDIUM COMPOSITION IN CELL SUSPENSION CULTURES OF Silybum marianum (L.) GAERTN.
Journal of Plant Production
Article 14, Volume 1, Issue 2, February 2010, Page 319-332 PDF (498.36 K)
Document Type: Original Article
DOI: 10.21608/jpp.2010.86356
View on SCiNiTO
Authors
Kawthar A. E. Rabie; Mona S. Abd El-Aal; H. H. Manaf
Agric. Botany Dept., Fac. of Agric., Ain Shams Univ., Shoubra El-Keima, Cairo, Egypt.
Abstract
Silymarin production was examined in cell suspension cultures derived from hypocotyl of Silybum marianum (L.) Gaertn. MS medium supplemented with different concentrations of sucrose, glucose, KH₂PO₄, KH₂PO₄ + K₂SO₄, NH₄NO₃, CaCl₂, L-Phenylalanine, L-Tyrosine and yeast extract was used. Tyrosine 10 mg/l, 0.5 KH₂PO₄ + K₂SO₄ con., 0.75 NH₄NO₃ con., 0.75 KH₂PO₄ + K₂SO₄ con., glucose 30 g/l, 0.5 CaCl₂.2H₂O, tyrosine 20 mg/l and yeast extract 40 mg/l (3 days incubation period) showed the highest production of silymarin in callus cells to reach 79%, 57.5%, 46%, 24.2%, 21.2%, 19.7%, 9.7% and 8.6% over the control respectively. The same trend was obtained by HPLC (qualitative assay).
Keywords
Silybum marianum; Silymarin; Cell suspension cultures


Statistics Article View: 87 PDF Download: 270