ACUTE TOXICITY OF SOME BIOPESTICIDES AND THEIR EFFECT ON ACETYLCHOLINESTERASE OF HONEY BEE (Apis mellifera) WORKERS
Eid, K., Marei, G., Abd-Elrasol, M. (2011). ACUTE TOXICITY OF SOME BIOPESTICIDES AND THEIR EFFECT ON ACETYLCHOLINESTERASE OF HONEY BEE (Apis mellifera) WORKERS. EKB Journal Management System, 2(10), 805-827. doi: 10.21608/jppp.2011.86600
Kh. S. A. Eid; Gehan I. Kh. Marei; Mona A. Abd-Elrasol. "ACUTE TOXICITY OF SOME BIOPESTICIDES AND THEIR EFFECT ON ACETYLCHOLINESTERASE OF HONEY BEE (Apis mellifera) WORKERS". EKB Journal Management System, 2, 10, 2011, 805-827. doi: 10.21608/jppp.2011.86600
Eid, K., Marei, G., Abd-Elrasol, M. (2011). 'ACUTE TOXICITY OF SOME BIOPESTICIDES AND THEIR EFFECT ON ACETYLCHOLINESTERASE OF HONEY BEE (Apis mellifera) WORKERS', EKB Journal Management System, 2(10), pp. 805-827. doi: 10.21608/jppp.2011.86600
Eid, K., Marei, G., Abd-Elrasol, M. ACUTE TOXICITY OF SOME BIOPESTICIDES AND THEIR EFFECT ON ACETYLCHOLINESTERASE OF HONEY BEE (Apis mellifera) WORKERS. EKB Journal Management System, 2011; 2(10): 805-827. doi: 10.21608/jppp.2011.86600

ACUTE TOXICITY OF SOME BIOPESTICIDES AND THEIR EFFECT ON ACETYLCHOLINESTERASE OF HONEY BEE (Apis mellifera) WORKERS

Journal of Plant Protection and Pathology
Article 2, Volume 2, Issue 10, October 2011, Page 805-827  XML PDF (622.39 K)
Document Type: Original Article
DOI: 10.21608/jppp.2011.86600
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Authors
Kh. S. A. Eid; Gehan I. Kh. Marei; Mona A. Abd-Elrasol
Dept. of Pest Control and Environmental Protection, Fac. Agric., Damanhour University.
Abstract
Biopesticides are increasingly applied throughout Egypt. However, negative effects of these compounds on the honey bee (Apis mellifera L.), the most important pollinator for cultivated ecosystem, remained poorly investigated. The objective of our study was to evaluate the potential side effects of five biopesticides; bioarch(Bacillus megaterium), biofly(Beauveria bassiana), biozed (Trichoderma album), protikto BTK(Bacillus thuringiensis kurstaki),and spintor(spinosad)on mortality and acetylcholinesterase activity of honey bee workers. The mortality of treated workers was determined after 24 h of application, and the lethal concentrations that caused 50% mortalities (LC50) were estimated. The impacts on acetylcholinesterase (AChE) activity were determined in vivo after 24 and 48 h in head, thorax and abdomen of surviving bees. Our results indicated that spinosad showed the most toxic action to adult honey bee workers with LC50 of 11.60 mg L-1 followed by biozed with LC50 of 114.12 mg L-1, and lower degrees of toxicity were obtained with protikto (LC50 = 87,412 mg L-1), biofly (LC50 = 49,766 mg L-1) and bioarch (LC50 = 15,785 mg L-1). In addition, all tested biopesticides caused various degrees of inhibition in AchE activity of adult honey bee workers (after 24 h of application) with the body region and with concentration. On the contrary, there were different degrees of activation in AChE of head, thorax and abdomen obtained after 48 h of application with tested biopesticides, except bioarch, biofly and protikto were inhibitions in AChE activities of the abdomen were obtained
Keywords
Honey bee; Apis mellifera L; biopesticides; Bioarch; Bacillus megaterium; Biofly; Beauveria bassiana; Biozed; Trichoderma album; Protikto; Bacillus thuringiensis kurstaki; Spintor; Spinosad; toxicity and Acetylcholinesterase
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