EFFECTIVENESS OF BIO-GEL BASED POWDER FORMULATIONS OF BACTERIAL BIOCONTROL AGENTS IN CONTROLING ROOT ROT DISEASE OF BEAN CAUSED BY Sclerotium rolfsii | ||||
| Journal of Plant Protection and Pathology | ||||
| Article 3, Volume 1, Issue 1, January 2010, Page 21-33 PDF (404.43 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jppp.2010.86653 | ||||
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| Author | ||||
| G. A. Amer* | ||||
| Agric. Bot. Dept., Fac. of Agric., Minufiya Univ. , Shibin El-Kom , Egypt | ||||
| Abstract | ||||
| Natural polymers(biogel) along with inert carriers were used to formulate two antagonistic bacterial isolates of Pseudomonas fluorescens and Bacillus subtilis. Four different formulations i.e. biogel- vermiculite based powder (F1), biogel-talc based powder (F2), biogel- charcoal based powder (F3) and biogel wettable powder (F4) were developed. These formulations were tested for their shelf life efficiency as storage substrates of the two bioagents for 24 month and their ability to control root-rot disease of bean caused by Sclerotium rolfsii. Viability of P. fluorescens propagules in biogel- vermiculite based powder and biogel wettable powder was stable for up to 16 months and decreased sharply after 20 months .The populations in biogel-talc based powder and biogel- charcoal based powder were decreased beyond 1 year of storage. B.subtilis populations of viable propagules were stable for up to 2 years with minor differences in populations either in formulation types or the storage periods tested. Active colonization of bean rhizosphere was occurred by P. fluorescens and B. subtilis following seed coating or soil amendment with different formulations. The highest populationof Ps. fluorescens and B. subtilis, was found in seed coating treatment than soil amendment. Biogel- vermiculite based powder gave the highest rhizosphere population followed by biogel wettable powder for both bacteria. In general, seed coating treatment gave an obvious results than soil amendment in reducing root rot disease incidence. Seed coating with B. subtilis applied in biogel- vermiculite based powder was the most effective one in reducing the disease incidence . Soil treatment was more effective than seed coating in reducing the sclerotia formed by Sclerotium rolfsii. Soil treatment with B. subtilis applied in biogel- vermiculite based powder was the most one in reducing the number of sclerotia formed. However, Soil amendment with B, subtilis in biogel- vermiculite based powder was the most effective treatment than other formula. | ||||
| Keywords | ||||
| Bean; root rot; P. fluorescens; B. subtilis; formulation | ||||
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