Ultrastructure Analysis of Primary and Permanent teeth with Generalized Amelogenesis imperfecta; In-vitro study
Hamed, G., El-Sayed, W. (2020). Ultrastructure Analysis of Primary and Permanent teeth with Generalized Amelogenesis imperfecta; In-vitro study. EKB Journal Management System, 66(Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)), 1041-1048. doi: 10.21608/edj.2020.24006.1016
Ghada Mohammed said Hamed; Walid El-Sayed. "Ultrastructure Analysis of Primary and Permanent teeth with Generalized Amelogenesis imperfecta; In-vitro study". EKB Journal Management System, 66, Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), 2020, 1041-1048. doi: 10.21608/edj.2020.24006.1016
Hamed, G., El-Sayed, W. (2020). 'Ultrastructure Analysis of Primary and Permanent teeth with Generalized Amelogenesis imperfecta; In-vitro study', EKB Journal Management System, 66(Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)), pp. 1041-1048. doi: 10.21608/edj.2020.24006.1016
Hamed, G., El-Sayed, W. Ultrastructure Analysis of Primary and Permanent teeth with Generalized Amelogenesis imperfecta; In-vitro study. EKB Journal Management System, 2020; 66(Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)): 1041-1048. doi: 10.21608/edj.2020.24006.1016

Ultrastructure Analysis of Primary and Permanent teeth with Generalized Amelogenesis imperfecta; In-vitro study

Egyptian Dental Journal
Article 10, Volume 66, Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), April 2020, Page 1041-1048  XML PDF (715.37 K)
Document Type: Original Article
DOI: 10.21608/edj.2020.24006.1016
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Authors
Ghada Mohammed said Hamed email orcid 1; Walid El-Sayed2
1ORAL BIOLOGY, SUZE CANAL UNVERSITY
2Oral Biology Department, Suez Canal University- Ismailia, Egypt
Abstract
Abstract
Amelogenesis or enamel formation is a biomineralization process in which specialized epithelial derived cells, ameloblasts, secrete extracellular matrix which later will be mineralized. amelogenesis can be divided into two main stages: the secretory stage and the maturation stage. Objectives: This work aims to investigate the ultrastructure phenotype of permanent and deciduous teeth with generalized enamel defects. Material and Methods; Teeth whether deciduous or permanent (exfoliated or extracted for clinical reasons), were collected from affected individuals. Scanning Electron Microscopy Energy Dispersive X-Ray Spectroscopy techniques were applied. Enzyme digestions was done by using either α- chymotrypsin (Sigma-UK) or lipase (Sigma-UK). Results: Examination of sections of affected teeth using Scanning Electron Microscopy showed abnormal enamel rods that were covered by amorphous material. This pattern was consistent of both primary and secondary dentitions. Energy Dispersive X-Ray Spectroscopy showed increase carbon contents. α- chymotrypsin enzyme was the only enzyme capable for removing this amorphous material. Conclusion: Ultrastructure analysis of the affected teeth showed typical form of hypomaturation amelogenins imperfecta. The study also proved that this amorphous material is mainly proteinases in nature.
Keywords
Enamel; Scanning Electron Microscope; Energy Dispersive X-Ray Spectroscopy; Amelogenesis imprecate
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