EFFECT OF DIFFERENT DOSES OF VITAMIN D3 SUPPLEMENTATION ON MANDIBULAR BONE IN RATS
Abdel-khalek, R., Aly, H., Omar, S., El-Adawy, M. (2020). EFFECT OF DIFFERENT DOSES OF VITAMIN D3 SUPPLEMENTATION ON MANDIBULAR BONE IN RATS. EKB Journal Management System, 45(2), 32-37. doi: 10.21608/adjalexu.2020.86764
Radwa R. Abdel-khalek; Hanaa M. Aly; Samia S. Omar; Mounir M. El-Adawy. "EFFECT OF DIFFERENT DOSES OF VITAMIN D3 SUPPLEMENTATION ON MANDIBULAR BONE IN RATS". EKB Journal Management System, 45, 2, 2020, 32-37. doi: 10.21608/adjalexu.2020.86764
Abdel-khalek, R., Aly, H., Omar, S., El-Adawy, M. (2020). 'EFFECT OF DIFFERENT DOSES OF VITAMIN D3 SUPPLEMENTATION ON MANDIBULAR BONE IN RATS', EKB Journal Management System, 45(2), pp. 32-37. doi: 10.21608/adjalexu.2020.86764
Abdel-khalek, R., Aly, H., Omar, S., El-Adawy, M. EFFECT OF DIFFERENT DOSES OF VITAMIN D3 SUPPLEMENTATION ON MANDIBULAR BONE IN RATS. EKB Journal Management System, 2020; 45(2): 32-37. doi: 10.21608/adjalexu.2020.86764

EFFECT OF DIFFERENT DOSES OF VITAMIN D3 SUPPLEMENTATION ON MANDIBULAR BONE IN RATS

Alexandria Dental Journal
Article 6, Volume 45, Issue 2, August 2020, Page 32-37  XML PDF (948.13 K)
Document Type: Original Article
DOI: 10.21608/adjalexu.2020.86764
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Authors
Radwa R. Abdel-khalek email 1; Hanaa M. Aly2; Samia S. Omar2; Mounir M. El-Adawy3
1Demonstrator of Oral Biology, Faculty of Dentistry, Alexandria University.
2Professor of Oral Biology, Faculty of Dentistry, Alexandria University.
3Professor of Animal Nutrition, Faculty of Agriculture, Alexandria University
Abstract
Introduction: vitamin D is one of the most important biological regulators of calcium metabolism which ultimately promotes healthy bones. It is also helpful in reducing inflammation as well as enhancing immune functions and cell growth. Despite its importance, its deficiency is prevalent worldwide. Moreover, it is difficult to obtain this vitamin from diet because it is not present in many foods. Consequently, Vitamin D3 (cholecalciferol) supplementation is nowadays used worldwide to maintain optimal bone health. However, studies evaluating the effect of standard doses of vitamin D supplements on bone have been inconsistent. In addition, the effects of higher doses have not been revealed and needs further studies.
Aim of the study: to determine the effect of supplementation of varying doses of dietary vitamin D3 on the structure of mandibular bone in albino rats.
Materials and methods: Thirty adult female rats were randomly divided into three groups: Group I control, while Group II and Group III received daily dose of vitamin D3 (400 IU/Kg) and (1000 IU/Kg) respectively. All rats were fed on same diet. After 6 weeks animals were euthanized and their mandibles were dissected out. The effects of each dose of vitamin D3 on the mandibular bone structure were assessed by scanning electron microscope and energy dispersive X-ray microanalysis.
Results:After6 weeks, Group I control and Group II supplemented with 400 IU/day, showed loss of its normal cortical bone smoothness. Moreover, the trabecular bone showed wide bone marrow spaces with thin trabeculae. However, group III supplemented with 1000 IU/day, showed smoother and more uniform cortical bone surface with wider trabeculae and narrower marrow spaces compared to the other groups.
Conclusion: This study showedthatadministration of vitamin D3 (1000 IU/day) enhanced bone architecture in adult rats.However, the administration of 400 IU/day showed slight enhancement in bone structure.
Keywords
vitamin D3; Cholecalciferol; Bone architecture; scanning electron microscope
References
  1. Tangpricha V, Koutkia P, Rieke SM, Chen TC, Perez AA, Holick MF. Fortification of orange juice with vitamin D: a novel approach for enhancing vitamin D nutritional health. Am J Clin Nutr. 2003;77:1478–83.
  2. Ross AC, Manson JE, Abrams SA, Aloia JF, Brannon PM, Clinton SK, et al. The 2011 Report on Dietary Reference Intakes for Calcium and Vitamin D from the Institute of Medicine. J Clin Endocrinol Metab. 2011;96:53–8.
  3. Hossein-nezhad A, Holick MF. Vitamin D for Health: A Global Perspective. Mayo Clin Proc. 2013;88:720–55.
  4. Pludowski P, Holick MF, Grant WB, Konstantynowicz J, Mascarenhas MR, Povoroznyuk V, et al. Vitamin D supplementation guidelines. J Steroid Biochem Mol Biol. 2018;175:125–35.
  5. Catharine Ross, Taylor CL, Ann L. Yaktine, B. H, Del Valle. Dietary Reference Intakes for Calcium and Vitamin D. Dietary References Intake For Vitamin D and Calcuim Food Nutrition Board. Washington, D.C.: National Academies Press; 2011.
  6. Norman AW, Bouillon R, Whiting SJ, Veith R, Lips P. 13th workshop consensus for vitamin D nutritional guidelines. J Steroid Biochem Mol Biol. 2007;103:204.
  7. Iheanacho I, Hart S, Greenhough G BH. Primary vitamin D deficiency in children. Drug Ther Bull. 2006 Feb;44:12–6.
  8. Sanders KM, Stuart AL, Williamson EJ, Simpson JA, Kotowicz MA, Young D, et al. Annual High-Dose Oral Vitamin D and Falls and Fractures in Older Women. JAMA. 2010;303:1815–22.
  9. Bischoff-Ferrari HA, Willett WC, Wong JB, Giovannucci E, Dietrich T, Dawson-Hughes B. Fracture prevention with vitamin D supplementation: A metaanalysis of randomized controlled trials. J Am Med Assoc. 2005;293:2257–64.
  10. Andersen R, Molgaard C, Skovgaard LT, Brot C, Cashman KD, Jakobsen J, et al. Effect of vitamin D supplementation on bone and vitamin D status among Pakistani immigrants in Denmark: a randomised doubleblinded placebo-controlled intervention study. Br J Nutr. 2008;100:197–207.
  11. Gorham ED, Garland CF, Garland FC, Grant WB, Mohr SB, Lipkin M, et al. Optimal Vitamin D Status for Colorectal Cancer Prevention. Am J Prev Med. 2007;32:210–6.
  12. Vieth R, Bischoff-Ferrari H, Boucher BJ. The urgent need to recommend an intake of vitamin D that is effective. Am J Clin Nutr. 2007;88:649–50.
  13. Reid IR, Bolland MJ, Grey A. Effects of vitamin D supplements on bone mineral density: a systematic review and meta-analysis. Lancet. 2014;383:146–55.
  14. Williamson L, Hayes A, Hanson ED, Pivonka P, Sims NA, Gooi JH. High dose dietary vitamin D 3 increases bone mass and strength in mice. Bone Reports. 2017;6:44–50.
  15. von Wowern N. General and oral aspects of osteoporosis: a review. Clin Oral Investig. 2001 Jun 1;5:71–82.
  16. Monje A, Chan H-L, Galindo-Moreno P, Elnayef B, Suarez-Lopez del Amo F, Wang F, et al. Alveolar Bone Architecture: A Systematic Review and Meta-Analysis. J Periodontol. 2015 Nov 1;86:1231–48.
  17. Pop LC, Sukumar D, Schneider SH, Schlussel Y, Stahl T, Gordon C, et al. Three doses of vitamin D, bone mineral density, and geometry in older women during modest weight control in a 1-year randomized controlled trial. Osteoporos Int. 2017;28:377–88.
  18. Cooper L, Clifton-Bligh PPB, Nery ML, Figtree G, Twigg S, Hibbert E, et al. Vitamin D supplementation and bone mineral density in early postmenopausal women. Am J Clin Nutr. 2003;77:1324–9.
  19. Mocanu V, Mocanu V, Stitt P a, Stitt P a, Costan AR, Costan AR, et al. Long-term effects of giving nursing home residents bread fortified with vitamind D per daily serving. Am J Clin Nutr. 2009;25(6):1132–7.
  20. Abulmeaty MMA. Sunlight exposure vs. vitamin D supplementation on bone homeostasis of vitamin D deficient rats. Clin Nutr Exp. 2017;11:1–9. 
  21. Kirkpatrick LA FB. A simple guide to IBM SPSS statistics for version 20.0.Student ed. Belmont, Calif.: Wadsworth, Cengage Learning; 2013.
  22. Larsen ER, Mosekilde L, Foldspang A. Vitamin D and Calcium Supplementation Prevents Osteoporotic Fractures in Elderly Community Dwelling Residents: A Pragmatic Population-Based 3-Year Intervention Study. J Bone Miner Res. 2003 Dec 22;19(3):370–8.
  23. Tang BM, Eslick GD, Nowson C, Smith C, Bensoussan A. Use of calcium or calcium in combination with vitamin D supplementation to prevent fractures and bone loss in people aged 50 years and older. Lancet. 2007;370:657–66.
  24. Smith H, Anderson F, Raphael H, Maslin P, Crozier S, Cooper C. Effect of annual intramuscular vitamin D on fracture risk in elderly men and women - A populationbased, randomized, double-blind, placebo-controlled trial. Rheumatology. 2007;46(12):1852–7.
  25. Michaelsson K, Melhus H, Bellocco R, Wolk A, Michae K, Melhus H, et al. Dietary calcium and vitamin D intake in relation to osteoporotic fracture risk. Bone. 2003;32(6):694–703.
  26. Armas LAG, Hollis BW, Heaney RP. Vitamin D2 is much less effective than vitamin D3 in humans. J Clin Endocrinol Metab. 2004;89(11):5387–91.
  27. Trang HM, Cole DE, Rubin LA, Pierratos A, Siu S, Vieth R. Evidence that vitamin D3 increases serum 25- hydroxyvitamin D more efficiently than does vitamin D2. Am J Clin Nutr. 1998;68(4):854–8.
  28. Grossmann RE, Tangpricha V. Evaluation of vehicle substances on vitamin D bioavailability: A systematic review. Mol Nutr Food Res. 2010 Apr 27;54:1055–61.
  29. Wolf E, Utech M, Stehle P, Büsing M, Helfrich H-P, Stoffel-Wagner B, et al. Oral High-Dose Vitamin D Dissolved in Oil Raised Serum 25-Hydroxy-Vitamin D to Physiological Levels in Obese Patients After Sleeve Gastrectomy—A Double-Blind, Randomized, and Placebo-Controlled Trial. Obes Surg. 2016 Aug 24;26:1821–9.
  30. Lee A, Anderson P, Sawyer R, Moore AJ, Forwood MR, Steck R, et al. Discordant effects of vitamin D deficiency in trabecular and cortical bone architecture and strength in growing rodents. J Steroid Biochem Mol Biol. 2010;121:284–7.
  31. Eisman JA, Bouillon R. Vitamin D: direct effects of vitamin D metabolites on bone: lessons from genetically modified mice. Bonekey Rep. 2014;3:499.
  32. El-Hajj Fuleihan G, Nabulsi M, Tamim H, Maalouf J, Salamoun M, Khalife H, et al. Effect of vitamin D replacement on musculoskeletal parameters in school children: a randomized controlled trial. J Clin Endocrinol Metab. 2006;91:405–12.
  33. Viljakainen HT, Natri A-M, Kärkkäinen M, Huttunen MM, Palssa A, Jakobsen J, et al. A positive doseresponse effect of vitamin D supplementation on sitespecific bone mineral augmentation in adolescent girls: a double-blinded randomized placebo-controlled 1-year intervention. J Bone Miner Res. 2006;21:836–44.
  34. Matsumoto T, Takano T, Yamakido S, Takahashi F, Tsuji N. Comparison of the effects of eldecalcitol and alfacalcidol on bone and calcium metabolism. J Steroid Biochem Mol Biol. 2010 Jul;121:261–4.
  35. Winzenberg T, Powell S, Shaw KA, Jones G. Effects of vitamin D supplementation on bone density in healthy children: systematic review and meta-analysis. BMJ. 2011 Jan;342. 
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