Characterization and Chemical Control of Neopestalotiopsis rosae the Causal Agent of Strawberry Root and Crown Rot in Egypt
Essa, T., Kamel, S., Ismail, A., El-Ganainy, S. (2018). Characterization and Chemical Control of Neopestalotiopsis rosae the Causal Agent of Strawberry Root and Crown Rot in Egypt. EKB Journal Management System, 46(1), 1-19. doi: 10.21608/ejp.2018.87411
Tarek A. Essa; Said M. Kamel; Ahmed M Ismail; Sherif El-Ganainy. "Characterization and Chemical Control of Neopestalotiopsis rosae the Causal Agent of Strawberry Root and Crown Rot in Egypt". EKB Journal Management System, 46, 1, 2018, 1-19. doi: 10.21608/ejp.2018.87411
Essa, T., Kamel, S., Ismail, A., El-Ganainy, S. (2018). 'Characterization and Chemical Control of Neopestalotiopsis rosae the Causal Agent of Strawberry Root and Crown Rot in Egypt', EKB Journal Management System, 46(1), pp. 1-19. doi: 10.21608/ejp.2018.87411
Essa, T., Kamel, S., Ismail, A., El-Ganainy, S. Characterization and Chemical Control of Neopestalotiopsis rosae the Causal Agent of Strawberry Root and Crown Rot in Egypt. EKB Journal Management System, 2018; 46(1): 1-19. doi: 10.21608/ejp.2018.87411

Characterization and Chemical Control of Neopestalotiopsis rosae the Causal Agent of Strawberry Root and Crown Rot in Egypt

Egyptian Journal of Phytopathology
Article 1, Volume 46, Issue 1, June 2018, Page 1-19  XML PDF (1010.88 K)
Document Type: Original Article
DOI: 10.21608/ejp.2018.87411
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Authors
Tarek A. Essa email orcid 1; Said M. Kamelorcid 1; Ahmed M Ismailorcid 1; Sherif El-Ganainy2
1Plant Pathology Research Institute, Agricultural Research Center, 12619, Giza, Egypt
2Plant Pests and Diseases Unit, College of Agricultural & Food Sciences, King Faisal Uni, Saudi Arabia
Abstract
Strawberry (Fragaria x ananassa Duch.) is a widely grown vegetable crop in Egypt. During a routine survey,wilt and root rot symptoms were observed on strawberry plants cv. Festival. Samples were collected and isolation was done on potato dextrose agar (PDA) medium. The frequently isolated fungi were Pestalotiopsis spp. along with other associated fungi. Koch’s postulates were fulfilled against two strawberry cultivars Fortuna and Festival under greenhouse conditions. Pestalotiopsis fungal isolates were identified by amplification and sequencing of ITS, TEF-1α and β-tubulin gene regions. The BLASTn search of GenBank database revealed that all obtained sequences exhibited similarity of 99–100% to Neopestalotiopsis rosae. Four fungicides were evaluated against N. rosae through in vitro and in vivo trials. In vitro trials revealed that fungicides Thiram and Hymexazole inhibited the mycelial growth of N. rosae at concentrations 1000 and 1250 ppm, respectively. In vivo trials showed that Thiram reduced significantly disease severity of crown and root rot on both cultivars.
Keywords
Chemical control; Crown rot; Molecular characterization; Neopestalotiopsis rosae; Strawberry
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