Evaluation of Endophytes Isolated from Rice Leaves for Their Antifungal Activities Against Pyricularia oryzae Causative Blast Disease | ||||
| Egyptian Journal of Phytopathology | ||||
| Article 12, Volume 46, Issue 1, June 2018, Page 193-214 PDF (691.76 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejp.2018.87788 | ||||
 View on SCiNiTO | ||||
| Author | ||||
Mona M. Saleh  
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| Plant Pathology Research Institute, Agricultural Research Center, 12619, Giza, Egypt. | ||||
| Abstract | ||||
| Rice crop is attacked during its growing season by many fungal diseases. Blast disease, caused by Pyricularia oryzae (Magnaporthe grisea Hebert), is the major serious one. The aim of this work was to replace fungicide applications by ecofriendly and natural substances against the disease. Fifty endophyte isolates of different microorganisms were isolated from rice leaves at Sakha Agricultural Research Farm on five different isolation media. In vitro test was performed to screen the antifungal activity of these isolates against rice blast fungus. Of these, only five isolates showed highly antagonistic activity in vitro and in vivo experiments. The secondary antifungal metabolites of these endophyte isolates were extracted by TLC and tested for their efficiency against the pathogen in in vitro test. Two isolates were the most potent and identified as Streptomyces albidoflavus strain Emeranaa MK203832.1 and Bacillus subtillus. GCMS extract analysis for these two isolates identified 24 and 13 compounds, respectively. | ||||
| Keywords | ||||
| Antimicrobial compounds; Endophytes; GC-MS analysis; Pyricularia oryzae; Rice blast and TLC test | ||||
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