STUDIES ON GENETIC VARIATION OF FIELD COLLECTED PINK BOLLWORM, PECTINOPHORA GOSSYPIELLA (SAUNDERS) USING RAPD TECHNIQUE | ||||
| Journal of Plant Protection and Pathology | ||||
| Article 10, Volume 5, Issue 4, April 2014, Page 481-495 PDF (431.45 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jppp.2014.87939 | ||||
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| Author | ||||
| Laila Seleman | ||||
| 1Plant protection Research Institute, Agricultural Research Center,Dokki, Giza. | ||||
| Abstract | ||||
Five strains of the pink bollworm, Pectinophoragossypiella (Sanders)were used in the present study. The laboratory strain was used as a baseline in the molecular biology assays. Four strains were selected from natural populations; fields located in Menoufia, Gharbia, Dakalia and Kafel-Shiekh, Governorates. The molecular studies included the analysis of the plod genomic DNA of the tested strains under this study by using RAPD-PCR method. A battery of five primers was used to evaluate the mutagenic among the sex strains. One primer (C07) generated the highest numbers of fragments, in which the fragments were 25. Four primers (B20, C02, C05 and E07) generated 20, 14, 20 and 17 fragments; respectively. The molecular sizes ranged between.152.891 and 1979.767bp. The RAPD patterns resulted from amplification of DNA of the field colony strains and laboratory strain of the pink bollworm, P. gossypiella revealed that the lowest value of similarity index was (0.0%), which reflects the highest degree of change in DNA structure and sequence between the genomes of untreated pink and those exposed to a wide spread of different insecticides used for controlling the pest in the fields. On the other hand, the four primers B20 and C02, recorded similarity index 1.0 between the laboratory strain and Dakahlia&Kafrel-Sheikh field colony strains; respectively. The primer C02 recorded similarity index 1.0 between Menoufia and Gharbia field colony strains. The primer C05 recorded similarity index 1.0 between Gharbia and Dakahlia field colony strains. Also, the primer C07 recorded similarity index 1.0 between Gharbia and Kafrel-Sheikh field colony strains. It is interest to note that the less damaging effect to pink bollworm DNA could be attributed to a good detoxifying mechanism developed by the insect as a result of wide spread and long term exposure of insect larvae in additional to different insecticides used in the fields. | ||||
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