CHITOSAN NANOPARTICLES PREPARED FROM LUCILIA CUPRINA MAGGOTS AS ANTIBACTERIAL AGENT | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Article 7, Volume 46, Issue 3, December 2016, Page 519-526 PDF (991.77 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jesp.2016.88251 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| MOSTAFA I. HASSAN1; FATMA A. TAHER2; ALY F. MOHAMED3; MOHAMMAD R. KAMEL1 | ||||
| 1Department of Zoology and Entomology, Faculty of Science, Al-Azhar University, Nasr City | ||||
| 2Department of Chemistry, Faculty of Science (Girls), Al-Azhar University, Nasr City | ||||
| 3Applied Researches Sector, Vacsera, Giza, Egypt | ||||
| Abstract | ||||
| Chitosan were prepared from cuticle of Lucilia cuprina maggots with two steps; deproteinization and deacetylation. It was characterized with solubility and Fourier Transform Infrared spectroscopy (FT-IR). Chitosan was ball-milled to obtain the chitosan nanoparticles which characterized with dynamic light scattering (DLS) and transmission electron microscope (TEM). Chitosan nanoparticles with degree of deacetylation (DDA) 80.5% were showed antibacterial activities against Klebsiella pneumoniae and Bacillus subtilis. The mode of action of chitosan nanoparticles on the tested bacteria was studied by TEM. Leakage of some cell contents, cell deformation and rupture of cell were observed, therefore, the chitosan nanoparticles were observed to be a powerful antibacterial agent. | ||||
| Keywords | ||||
| Antibacterial agent; Chitosan nanoparticles; Lucilia cuprina; Maggots | ||||
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