EFFECT OF GARDEN CRESS ON ALVEOLAR BONE HEALING IN RATS WITH LIGATURE INDUCED PERIODONTITIS (Histological& Ultrastructural study)
Salem, E., Karam, S., Kawana, K., Khalil, N., Lotfy, M. (2020). EFFECT OF GARDEN CRESS ON ALVEOLAR BONE HEALING IN RATS WITH LIGATURE INDUCED PERIODONTITIS (Histological& Ultrastructural study). EKB Journal Management System, 45(2), 79-83. doi: 10.21608/adjalexu.2020.88442
Eman M. Salem; Sahar S. Karam; Khadiga Y. Kawana; Nesma M. Khalil; Mona E. Lotfy. "EFFECT OF GARDEN CRESS ON ALVEOLAR BONE HEALING IN RATS WITH LIGATURE INDUCED PERIODONTITIS (Histological& Ultrastructural study)". EKB Journal Management System, 45, 2, 2020, 79-83. doi: 10.21608/adjalexu.2020.88442
Salem, E., Karam, S., Kawana, K., Khalil, N., Lotfy, M. (2020). 'EFFECT OF GARDEN CRESS ON ALVEOLAR BONE HEALING IN RATS WITH LIGATURE INDUCED PERIODONTITIS (Histological& Ultrastructural study)', EKB Journal Management System, 45(2), pp. 79-83. doi: 10.21608/adjalexu.2020.88442
Salem, E., Karam, S., Kawana, K., Khalil, N., Lotfy, M. EFFECT OF GARDEN CRESS ON ALVEOLAR BONE HEALING IN RATS WITH LIGATURE INDUCED PERIODONTITIS (Histological& Ultrastructural study). EKB Journal Management System, 2020; 45(2): 79-83. doi: 10.21608/adjalexu.2020.88442

EFFECT OF GARDEN CRESS ON ALVEOLAR BONE HEALING IN RATS WITH LIGATURE INDUCED PERIODONTITIS (Histological& Ultrastructural study)

Alexandria Dental Journal
Article 13, Volume 45, Issue 2, August 2020, Page 79-83  XML PDF (740.18 K)
Document Type: Original Article
DOI: 10.21608/adjalexu.2020.88442
View on SCiNiTO View on SCiNiTO
Authors
Eman M. Salem email 1; Sahar S. Karam2; Khadiga Y. Kawana2; Nesma M. Khalil3; Mona E. Lotfy4
1Assistant lecturer of Oral Biology, Faculty of Dentistry, Pharos University, Alexandria, Egypt.
2Professor of Oral Biology, Faculty of Dentistry, Alexandria University, Alexandria.
3Lecturer of Oral Biology, Faculty of Dentistry, Alexandria University, Alexandria Egypt.
4Professor of periodontology, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
Abstract
Introduction: Lepidium sativum, commonly known as garden cress is a herb that is native to Egypt and West Asia. Its seeds are rich source of proteins, dietary fiber, omega-3 fatty acids, iron, other essential nutrients and phytochemicals. Garden cress is widely used in folk medicine for the treatment of many diseases. Periodontitis is defined as an inflammatory bacterial-induced disease that damages the soft tissue and gradually destroys the periodontal ligaments and supporting alveolar bone. Periodontitis can cause teeth to loosen or lead to tooth loss. Different methods, such asbiomechanical measurements, surgical approaches are used to treat periodontitis.
Objective: to evaluate histologically and ultra-structurally the possible effect of garden cress therapy on alveolar bone healing in rats with ligature induced periodontitis.
Materials and Methods: Fortyfive adult male Sprague–Dawley rats of 6 months old (200-250 grams in weight) was used in this study. They were divided into 3 equal groups: group A; (control group), group B; (ligature induced periodontitis) and group C :( ligature induced periodontitis with garden cress administration). Ligature was removed after 11 days. Garden cress was applied after ligature removal systemically by using oral gavage. Rats were sacrificed after six weeks from the ligature removal. Mandibles were dissected out and were prepared for histological and scanning electron microscopic evaluation.
Results: After 6 weeks the specimens of group B (periodontitis group) showed alveolar bone loss, while in group C (garden cress group) showed relative restoration of alveolar bone. Results of scanning electron microscope revealed an irregular & porous bone surface of group B, while Group C showed relative smooth and regular alveolar bone.
Conclusion: Garden Cress systemic administration accelerates alveolar bone healing and enhances bone formation in periodontal diseases.
Keywords
Garden Cress; periodontitis; alveolar bone; rats
References
  1. Rafińska K, Pomastowski P, Rudnicka J, Krakowska A, Maruśka A, Narkute M, et al. Effect of solvent and extraction technique on composition and biological activity of Lepidium sativum extracts. Food Chem. 2019; 289:16-25.
  2. Altay A, Koktepe T, Durmaz L, Topal F, Gulcin I. Purification and selected biochemical properties of peroxidase from cress (Lepidium sativum sub sp. sativum)." Int J Food Prop. 2018;21: 2610-21.
  3. Washio K, Tsutsumi Y, Tsumanuma Y, Yano K, Srithanyarat SS, Takagi R, et al. In vivo periodontium formation around titanium implants using periodontal ligament cell sheet. Tissue Eng Part A. 2018; 24:1273- 82.
  4. Kishor C, Mishra RR, Saraf SK, Kumar M, Srivastav AK, Nath G. Phage therapy of staphylococcal chronic osteomyelitis in experimental animal model. Indian J Med Res. 2016; 143:87-94.
  5. Florencio-Silva R, Sasso GR, Sasso-Cerri E, Simões MJ, Cerri PS. Biology of bone tissue: structure, function, and factors that influence bone cells. Biomed Res Int. 2015; 2015:421746.
  6. Hajishengallis G. Periodontitis: from microbial immune subversion to systemic inflammation. Nat Rev Immunol. 2015; 15:30-44.
  7. Longo M, Gouveia Garcia V, Ervolino E, Ferro Alves ML, Duque C, Wainwright M, et al. Multiple aPDT sessions on periodontitis in rats treated with chemotherapy: histomorphometrical, immunohistochemical, immunological and microbiological analyses. Photodiagnosis Photodyn Ther. 2019; 25:92-102.
  8. O'Boyle C, Haley MJ, Lemarchand E, Smith CJ, Allan SM, Konkel JE, et al. Ligature-induced periodontitis induces systemic inflammation but does not alter acute outcome after stroke in rats. Int J Stroke. 2019:174749301983419.
  9. Feldman AT, Wolfe D. Tissue processing and hematoxylin and eosin staining. Methods Mol Biol. 2014; 1180:31-43.
  10. Murtey MD, Ramasamy P. Sample Preparations for Scanning Electron Microscopy–Life Sciences. In: Janecek M (ed). Modern Electron Microscopy in Physical and Life Sciences. Intech; 2016.
  11. Tonetti MS, Greenwell H, Kornman KS. Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. J Periodontol. 2018;89 (Suppl 1): S159-72.
  12. Manji F, Dahlen G, Fejerskov O. Caries and periodontitis: contesting the conventional wisdom on their aetiology. Caries Res. 2018; 52:548-564.
  13. Slots J. Periodontitis: facts, fallacies and the future. Periodontol 2000 2017; 75:7-23.
  14. Kissa J, Chemlali S, El Houari B, Amine K, Khlil N, Mikou S, et al. Aggressive and chronic periodontitis in a population of Moroccan school students. J Clin Periodontol. 2016; 43:934-9.
  15. Mali RG., Shailaja G. "Lepidium sativum (Garden cress): a review of contemporary literature and medicinal properties.Oriental Pharmacy and Experimental Medicine 2007: 331-335.
  16. Juma AB. The effects of Lepidium sativum seeds on fracture-induced healing in rabbits. MedGenMed. 2007; 9:23.
  17. Bukhari HM, Wahb HM, Header EA, Sharkar AA, Alsaegh NA, Bukhari SM, et al. Effects of Lepidium Sativum (Tuffa) on Bone Mineral Density and Symptoms in Female University Students in Makkah, Saudi Arabia: Single-Blind Randomized Trail. Am J Food Nutr Res. 2018; 5:48-54.
  18. Imade OV, Smith O F, Gazal OS. Effects of dietary inclusion of Lepidium sativum (garden cress) seed on plasma luteinizing hormone and reproductive performance in female rabbits. Afr Assoc Physiol Sci. 2018; 6:79-84.
Statistics
Article View: 385
PDF Download: 699