EFFECT OF PHYTIC ACID (IP6) VERSUS ETHYLENE DIAMINE TETRA ACETIC ACID (EDTA) ON DENTIN MICROHARDNESS (IN VITRO STUDY) | ||||
| Alexandria Dental Journal | ||||
| Article 4, Volume 46, Issue 2, August 2021, Page 99-105 PDF (1.11 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/adjalexu.2020.88460 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mohamed M. Naeem  1; Amr M. Abdallah2; Adel A. Kamar3; Nihal A. Leheta4
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| 1Bachelor of Dentistry, Conservative Dentistry Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt | ||||
| 2Professor of Endodontics, Conservative Dentistry Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt. | ||||
| 3Professor of Dental Biomaterials, Dental Biomaterials Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt. | ||||
| 4Lecturer of Endodontics Conservative Dentistry Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt. | ||||
| Abstract | ||||
| Introduction: The use of chelating agents removes smear layer, increasing the access of irrigants to allow disinfection, and also reduces microhardness. Microhardness is an indirect evidence of mineral changes in dentin. Its reduction facilitates the instrumentation throughout the canal but, it may weaken the root and increases permeability of dentin. Therefore, finding a biocompatible chelating agent with better chelating ability is mandatory. Objective: To evaluate the effect Phytic acid (IP6) with different concentrations and ethylene diamine tetra-acetic acid (EDTA) solution on microhardness of radicular dentin. Materials and method: This study was conducted on 30 single rooted permanentmaxillary anteriors. Teeth were instrumented using rotary files and irrigated in between using 2ml 2.5% NaOCl, then were sectioned longitudinally into halves giving 60 specimens that were embedded in acrylic resin leaving the dentin exposed. After polishing, the microhardness values of the untreated dentin were recorded by Vicker’s microhardness tester. The root halves were divided into 4 groups composed of 15 samples each and immersed for 5 minutes with one of the chelating solutions, Group I: Immersed in 10ml of (IP6) 0.5% followed by 10ml of 2.5% (NaOCl), Group II: Immersed in 10ml of (IP6) 1% followed by 10ml of 2.5% NaOCl, Group III: Immersed in 10ml of 1.5% (IP6) followed by 10ml of 2.5% NaOCl, Group IV: Immersed in 10ml of 17% EDTA solution followed by 10ml of 2.5% NaOCl. Then microhardness values were recorded, and calculation of the difference between baseline values and post-application values were calculated. Results: Tested chelators reduced microhardness of the dentin. 17% EDTA reduced the microhardness more significantly than 0.5%, 1% and 1.5% IP6. Conclusion: Using IP6 with different concentrations had less detrimental effect on dentin microhardness than EDTA. | ||||
| Keywords | ||||
| Phytic acid; EDTA; smear layer; dentin microhardness | ||||
| References | ||||
|
1. Virdee SS, Seymour DW, Farnell D, Bhamra G, Bhakta S. Efficacy of irrigant activation techniques in removing intracanal smear layer and debris from mature permanent teeth: a systematic review and meta-analysis. Int Endod J 2018;51:605-21.
2. Calt S, Serper A. Time dependent effects of EDTA on dentine structures. J Endod 2002; 28:17-9.
3. Cruz-Filho AM, Sousa-Neto MD, Savioli RN, Silva RG, Vansan LP, Pécora JD. Effect of chelating solutions on the microhardness of root canal lumen dentin. J Endod 2011;37:358-62.
4. Amaral KF, Rogero MM, Fock RA, Borelli P, Gavini G. Cytotoxicity analysis of EDTA and citric acid applied on murine resident macrophages culture. Int Endod J. 2007;40:338-43.
5. Nassar M, Hiraishi N, Tamura Y, Otsuki M, Aoki K, Tagami J. Phytic Acid: An Alternative Root Canal Chelating Agent. J Endod. 2015;41:242-7.
6. Kandil HE, Labib AH, Alhadainy A. Effect of different irrigant solutions on microhardness and smear layer removal of root canal dentin. Tanta Dent J. 2014;1:1-11.
7. Cruz-FilhoAM, Sousa-Neto MD, Saquy PC, Pécora JD. Evaluation of the effect of EDTAC, CDTA, and EGTA on radicular dentin microhardness. J Endod. 2001;27:183-4.
8. Ulusoy Öİ, Görgül G. Effects of different irrigation solutions on root dentin microhardness, smear layer removal and erosion. Aust Endod J. 2013;39:66-72.
9. Voort GFV, Lucas GM. Microindentation Hardness Testing. Adv Mater Proc.1998;154:21-5.
10. Sayin TC, Serper A, Cehreli ZC, Otlu HG. The effect of EDTA, EGTA, EDTAC and tetracycline-HCL with and without subsequent NaOCl treatment on the microhardness of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007;104:418-24.
11. Akcay I, Sen BH. The effect of surfactant addition to EDTA on microhardness of root dentin. J Endod. 2012;38:704-7.
12. Teixeira CS, Felippe MCS, Felippe WT. The effect of application time of EDTA and NaOCl on intracanal smear layer removal: an SEM analysis. Int Endod J. 2005;38:285-90.
13. Rotstein I, Dankner E, Goldman A, Heling I, Stabholz A, Zalkind M. Histochemical analysis of dental hard tissues following bleaching. J Endod. 1996;22:23-5.
14. Craig RG, Gehring PE, Peyton FA. Relation of structure to the microhardness of human dentin. J Dent Res. 1959;38:624-30.
15. Ari H, Erdemir A. Effects of endodontic irrigation solutions on mineral content of root canal dentin using ICP-AES technique. J Endod. 2005;31:187-9.
16. Lopes MB, Sinhoreti MA, GoniniJúnior A, Consani S, McCabe JF. Comparative study of tubular diameter and quantity for human and bovine dentin at different depths. Braz Dent J. 2009; 20: 279-83.
17. Weshah MM. In vitro effect of 2% chlorhexidine on dentin Microhardnes. PODJ. 2011;31:173-7.
18. Ossareh A, Kishen A. Role of dentin compositional changes and structural loss on fracture predilection in endodontically treated teeth. M.Sc. Thesis. Graduate Department of Dentistry, University of Toronto. 2015. p. 1-79.
19. Baghdadi R, Hassanein I. Effect of different irrigants on same mechanical properties of dentin an attempt to improve fracture strength and microhardness of endodontically treated teeth. Egypt Dent J. 2004;50:199-208.
20. Weshah MM. In vitro effect of 2% chlorhexidine on dentin Microhardnes. PODJ. 2011;31:173-7.
21. Jefferson JC, Manhães FC, Bajo H, Duque TM. Efficiency of different concentrations of sodium hypochlorite during endodontic treatment. Dental Press Endod J. 2012;2:32-7.
22. Aranda-Garcia AJ, Kuga MC, Chavéz-Andrade GM, Kalatzis-Sousa NG, Hungaro Duarte MA, Faria G, et al. Effect of final irrigation protocols on microhardness and erosion of root canal dentin. Microsc Res Tech. 2013;76:1079-83.
23. Saghiri MA, Delvarani A, Mehrvarzfar P, Malganji G, Lotfi M, Dadresanfar B, et al. A study of the relation between erosion and microhardness of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;108:29-34.
24. Patil CR, Uppin V. Effect of endodontic irrigating solutions on the microhardness and roughness of root canal dentin: An in vitro study. Ind J Dent Res. 2011;22:22-7.
25. Chuenarrom C, Benjakul P, Daosodsai P. Effect of indentation load and time on Knoop and Vickers Microhardness tests for enamel and dentin. Mat Res. 2009;12:473-6.
26. Voort GFV. Hardness. In: Metallography, Principles and Practice. 4th ed. USA: Technology & Engineering; 2007. p. 334-82.
27. Gutiérrez-Salazar M, Reyes-Gasga J. Microhardness and chemical composition of human tooth. Mat Res. 2003;6:367-73.
28. Fuentes V, Toledano M, Osorio R, Carvalho RM. Microhardness of superficial and deep sound human dentin. J Biomed Mater Res A. 2003;66:850-3.
29. Saleh HAJ. Comparative Evaluation of Effect of Irrigation Solutions with Various Exposure Time on microhardness of Root Canal Dentin. Iraqi Dent J. 2016;38:124-8.
30. Cirano FR, Romito GA, Todescan JH. Determination of root dentin and cementum micro hardness. Braz J Oral Sci. 2004;3:420-4.
31. Al-Ashou WM. The Effects of Two Root Canal Irrigants and Different Instruments on Dentin Microhardness (In Vitro Study). Al–Rafidain Dent J. 2011;11:63-70.
32. Aslantas EE, Buzoglu HD, Altundasar E, Serper A. Effect of EDTA, sodium Hypochlorite, and chlorhexidinegluconate with or without surface modifiers on dentin microhardness. J Endod. 2014;40:876-97.
33. De-Deus G, Paciornik S, Mauricio MH. Evaluation of the effect of EDTA, EDTAC, and Citric acid on the microhardness of root dentin. Int Endod J. 2006;39:401-7.
34. Çalt S, Serper A. Time-dependent effects of EDTA on dentin structures. Endod J. 2002;28:17-9.
35. Paque F, Luder HU, Sener B, Zehnder M. Tubular sclerosis rather than the smear layer impededs dye penetration in to the dentin of endodontically instrumented root canals. Int Endod J. 2006;39:18-25.
36. Torabinejad M. Root Canal Irrigants and Disinfectants. Chicago, IL: American Association of Endodontists Colleagues for Excellence, Newsletter; 2011. p.1-7.
37. Haapasalo M, Shen Y, Qian W, Gao Y. Irrigation in endodontics. Dent Clin North Am. 2010;54:291-312.
38. Singh S, Acharya SR, Ballal V, Rijesh M. Evaluation of the effect of EDTA, EDTAC, RC-Prep and BioPure MTAD on the microhardness of root canal dentin -An in vitro study. Endodont. 2009;21:35-41.
39. Carrigan PL, Morse DR, Furst ML, Sinai IH. A scanning electron microscopic evaluation of human dentinal tubules according to age and location. J Endod. 1984;10:359-63.
40. Pashley D, Okabe A, Parham P. The relationship between dentin microhardness and tubule density. Endod Dent Traumatol. 1985;1:176-9.
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