Expression of Tolerogenic HLA-G Confer Worse Outcome in Patients with Chronic Lymphocytic Leukemia | ||||
The Egyptian Journal of Hospital Medicine | ||||
Article 6, Volume 80, Issue 1, July 2020, Page 587-593 PDF (712.01 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejhm.2020.88567 | ||||
View on SCiNiTO | ||||
Authors | ||||
Omar Elhenawy Mohamed Thabet 1; Wafaa Salah Mohamed1; Magda Assem2; Abdel Rahman Abdel Hamid3; Randa Amin Osman2 | ||||
1Department of Clinical Pathology, Faculty of Medicine, Aswan University | ||||
2Department of Clinical Pathology, National Cancer Institute, Cairo univeristy | ||||
3Department of Clinical Pathology, Faculty of Medicine, Qena University, Egypt | ||||
Abstract | ||||
Background: Chronic lymphocytic leukemia (CLL) is the most common hematological malignancy in adults presenting with varied clinical course. There is a higher request for founding known prognostic factors for stratifying CLL patients. HLA-G is a non-classical major histocompatibility complex (MHC) molecule. It can be expressed in membrane bound (m.HLA-G) and soluble forms (s.HLA-G). Objective: The aim of work was to investigate the expression of membrane form of HLA-G in CLL and correlate findings and a variety of clinical and laboratory variables. Patients and methods: This prospective study included a total of thirty newly diagnosed B-CLL patients, attending at Hematology Unit, Medical Oncology Department, national Cancer institute, Cairo. This study was conducted between December 2017 to July 2019. Diagnosis of CLL was confirmed by flow cytometric immunophenenotyping using standard lymphoma panel, membrane HLA-G was determined by flow cytometry. Results: The expression of HLA-G by flowcytometry was negatively correlated with the platelet count (r = -0.516, p = 0.004) and Hb (r = -0.479, p = 0.007). The expression of HLA-G was significantly higher in CD38 positive cells (p = 0.006). The expression of HLA-G was significantly higher in Rai stage 4 compared to stage 1 & stage 2 (p = 0.001). Conclusion: It could be concluded that expression of HLA-G might represent signs of immunosuppression in CLL patients which contribute to the immune escape of tumor cells. In addition, HLA-G expression by B-CLL cells correlates significantly with the known prognostic markers of disease progression, mainly Rai clinical staging, CD38 expression and worse survival, making this parameter possibly an important prognostic factors of disease progression. | ||||
Keywords | ||||
CLL; HLA-G | ||||
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