Biological, Serological and Molecular Characterization of Egyptian Zucchini Yellow Mosaic Virus Isolate Infecting Squash Plants in Fayoum Governorate
Kheder, A., Sulaiman, T., Ghanem, G., Tohamy, M. (2017). Biological, Serological and Molecular Characterization of Egyptian Zucchini Yellow Mosaic Virus Isolate Infecting Squash Plants in Fayoum Governorate. EKB Journal Management System, 45(2), 1-14. doi: 10.21608/ejp.2017.88570
Ahmed A. Kheder; Taqwa Sulaiman; Gamal Ghanem; Mohamed Tohamy. "Biological, Serological and Molecular Characterization of Egyptian Zucchini Yellow Mosaic Virus Isolate Infecting Squash Plants in Fayoum Governorate". EKB Journal Management System, 45, 2, 2017, 1-14. doi: 10.21608/ejp.2017.88570
Kheder, A., Sulaiman, T., Ghanem, G., Tohamy, M. (2017). 'Biological, Serological and Molecular Characterization of Egyptian Zucchini Yellow Mosaic Virus Isolate Infecting Squash Plants in Fayoum Governorate', EKB Journal Management System, 45(2), pp. 1-14. doi: 10.21608/ejp.2017.88570
Kheder, A., Sulaiman, T., Ghanem, G., Tohamy, M. Biological, Serological and Molecular Characterization of Egyptian Zucchini Yellow Mosaic Virus Isolate Infecting Squash Plants in Fayoum Governorate. EKB Journal Management System, 2017; 45(2): 1-14. doi: 10.21608/ejp.2017.88570

Biological, Serological and Molecular Characterization of Egyptian Zucchini Yellow Mosaic Virus Isolate Infecting Squash Plants in Fayoum Governorate

Egyptian Journal of Phytopathology
Article 1, Volume 45, Issue 2, December 2017, Page 1-14  XML PDF (853.53 K)
Document Type: Original Article
DOI: 10.21608/ejp.2017.88570
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Authors
Ahmed A. Kheder email orcid 1; Taqwa Sulaiman2; Gamal Ghanemorcid 3; Mohamed Tohamy4
1Plant Pathology Research Institute, Agricultural Research Center, 12619, Giza, Egypt
2M.Sc. Student, Inst. Asian Studies & Researches., Zagazig Univ
3Plant Pathol. Dept., Fac. Agric., Cairo Univ
4Plant Pathol. Dept., Fac. Agric., Zagazig Univ
Abstract
Zucchini yellow mosaic virus (ZYMV) is one of the most important viruses and is responsible for significant losses in cucurbit crops worldwide. In Egypt, it causes serious economic losses in squash especially in the spring season. During 2014-2015, samples were collected from squash fields in different Provinces, Fayoum governorate. Inspection in Fayoum, ZYMV-naturally infected squash plants exhibited severe mosaic and blisters, leaves deformation, blisters on the fruits and discoloration hardening of flesh, as well as external fruit cracks. Percentages of infection in squash cv. Eskandrani, ranged between 20-35% and 70-78% in mid-March and May, respectively. Meanwhile, it ranged between 15-25% in September and 62% in the end of November. The virus was isolated from naturally-infected squash plants, detected serologically depending on enzyme-linked immunosorbant assay (ELISA), and biologically by mechanical inoculation to different species belonging four families i.e., Amarnthaceae, Chenopodiaceae, Cucurbitaceae, Solanacea. In virus vector relationship, transmission rates recorded 84.6, 86.6, 93.3, 90, and 91.6%, respectively for squash cvs. Alia, Asma, Eskandrani, Mabrouka and Safa. In this context, one insect vector (Myzus persicae) proved its ability and efficacy to transmit ZYMV. Electron microscopy (EM) of partially purified preparation method revealed flexuous particles approximately 750X15 nm. Serologically, TBIA proved to be effective in detecting ZYMV in the infected samples and reliable technique for the virus detection in squash leaf midrib and petioles. Electrophoresis analysis of the reverse transcription polymerase chain reaction (RT-PCR) amplification revealed that the primers amplified a product size of 458bp for the RNA extracted from ZYMV-infected tissues.
Keywords
Myzus persicae; Polymerase chain reaction (PCR); RT-PCR; Zucchini yellow mosaic virus; ZYMV
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