IMMUNE-MOLECULAR IDENTIFICATION OF GIARDIA INTESTINALIS IN DIARRHOEAL CHILDREN: COMPARISON OF THREE DIAGNOSTIC METHODS
MADBOULY, N., FARID, A., EL-BADRY, A., EL-AMIR, A. (2016). IMMUNE-MOLECULAR IDENTIFICATION OF GIARDIA INTESTINALIS IN DIARRHOEAL CHILDREN: COMPARISON OF THREE DIAGNOSTIC METHODS. EKB Journal Management System, 46(2), 253-260. doi: 10.21608/jesp.2016.88648
NEVEEN A. MADBOULY; ALYA FARID; AYMAN A. EL-BADRY; AZZA M. EL-AMIR. "IMMUNE-MOLECULAR IDENTIFICATION OF GIARDIA INTESTINALIS IN DIARRHOEAL CHILDREN: COMPARISON OF THREE DIAGNOSTIC METHODS". EKB Journal Management System, 46, 2, 2016, 253-260. doi: 10.21608/jesp.2016.88648
MADBOULY, N., FARID, A., EL-BADRY, A., EL-AMIR, A. (2016). 'IMMUNE-MOLECULAR IDENTIFICATION OF GIARDIA INTESTINALIS IN DIARRHOEAL CHILDREN: COMPARISON OF THREE DIAGNOSTIC METHODS', EKB Journal Management System, 46(2), pp. 253-260. doi: 10.21608/jesp.2016.88648
MADBOULY, N., FARID, A., EL-BADRY, A., EL-AMIR, A. IMMUNE-MOLECULAR IDENTIFICATION OF GIARDIA INTESTINALIS IN DIARRHOEAL CHILDREN: COMPARISON OF THREE DIAGNOSTIC METHODS. EKB Journal Management System, 2016; 46(2): 253-260. doi: 10.21608/jesp.2016.88648

IMMUNE-MOLECULAR IDENTIFICATION OF GIARDIA INTESTINALIS IN DIARRHOEAL CHILDREN: COMPARISON OF THREE DIAGNOSTIC METHODS

Journal of the Egyptian Society of Parasitology
Article 3, Volume 46, Issue 2, August 2016, Page 253-260  XML PDF (3.94 MB)
Document Type: Original Article
DOI: 10.21608/jesp.2016.88648
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Authors
NEVEEN A. MADBOULY1; ALYA FARID1; AYMAN A. EL-BADRY2; AZZA M. EL-AMIR1
1Department of Zoology, Faculty of Science, Cairo University, Giza 12613, Egypt.
2Department of Medical Parasitology, Faculty of Medicine, Cairo University, Cairo, Egypt
Abstract
Giardiasis is a major health problem in both developed and developing world. A variety of methods for diagnosis of Giardia duodenalis cysts or trophozoites is available but still has certain limitations. 100 sample from diarrhoeal children who attending outpatient clinic in Abu El Rish hospital, Kasr Al Ainy, Faculty of Medicine, Cairo University, Egypt. Giardiasis was diagnosed by direct wet mount, microscopy after formal- ethyl acetate concentration, Ridascreen ELISA assay and n-PCR targeting beta giardin (bg) gene. Using ELISA as reference standard, the methods' sensitivities, specificities, positive (PPV) and negative (NPV) predictive values and positive (LR+) and negative (LR-) likelihood ratios with 95% confidence interval (95% CI) were analyzed.The diagnostic methods were evaluated to determine their impact on the posttest probability using Fagan’s nomogram. All the studied methods led to a LR+ higher than 10 indicating ability to ruling in giardiasis. n-PCR recorded LR- equal 0.00 and the probability of giardiasis would be 0% if the test was negative. The methods were also ranked on basis of Multiattribute utility theory and Analytical hierarchy process with ELISA ranked better than n-PCR.
Keywords
Giardia; Copro-DNA; bg gene; Ridascreen ELISA; Fagan's nomogram
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