LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP): SENSITIVE AND RAPID DETECTION OF SCHISTOSOMA HAEMATOBIUM DNA IN URINE SAMPLES OF EGYPTIAN SUSPECTED CASES.
BAYOUMI, A., AL-REFAI, S., BADR, M., ABD EL-AAL, A., EL AKKAD, D., SAAD, N., ELESAILY, K., ABDEL AZIZ, I. (2016). LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP): SENSITIVE AND RAPID DETECTION OF SCHISTOSOMA HAEMATOBIUM DNA IN URINE SAMPLES OF EGYPTIAN SUSPECTED CASES.. EKB Journal Management System, 46(2), 299-308. doi: 10.21608/jesp.2016.88677
A. BAYOUMI; S. A. AL-REFAI; M. S. BADR; A. A. ABD EL-AAL; D. M. H. EL AKKAD; NAGLAA SAAD; K. M. ELESAILY; I. Z. ABDEL AZIZ. "LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP): SENSITIVE AND RAPID DETECTION OF SCHISTOSOMA HAEMATOBIUM DNA IN URINE SAMPLES OF EGYPTIAN SUSPECTED CASES.". EKB Journal Management System, 46, 2, 2016, 299-308. doi: 10.21608/jesp.2016.88677
BAYOUMI, A., AL-REFAI, S., BADR, M., ABD EL-AAL, A., EL AKKAD, D., SAAD, N., ELESAILY, K., ABDEL AZIZ, I. (2016). 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP): SENSITIVE AND RAPID DETECTION OF SCHISTOSOMA HAEMATOBIUM DNA IN URINE SAMPLES OF EGYPTIAN SUSPECTED CASES.', EKB Journal Management System, 46(2), pp. 299-308. doi: 10.21608/jesp.2016.88677
BAYOUMI, A., AL-REFAI, S., BADR, M., ABD EL-AAL, A., EL AKKAD, D., SAAD, N., ELESAILY, K., ABDEL AZIZ, I. LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP): SENSITIVE AND RAPID DETECTION OF SCHISTOSOMA HAEMATOBIUM DNA IN URINE SAMPLES OF EGYPTIAN SUSPECTED CASES.. EKB Journal Management System, 2016; 46(2): 299-308. doi: 10.21608/jesp.2016.88677

LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP): SENSITIVE AND RAPID DETECTION OF SCHISTOSOMA HAEMATOBIUM DNA IN URINE SAMPLES OF EGYPTIAN SUSPECTED CASES.

Journal of the Egyptian Society of Parasitology
Article 6, Volume 46, Issue 2, August 2016, Page 299-308  XML PDF (4.82 MB)
Document Type: Original Article
DOI: 10.21608/jesp.2016.88677
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Authors
A. BAYOUMI1; S. A. AL-REFAI1; M. S. BADR2; A. A. ABD EL-AAL1; D. M. H. EL AKKAD1; NAGLAA SAAD1; K. M. ELESAILY3; I. Z. ABDEL AZIZ1
1Medical Parasitology, Faculty of Medicine, Cairo University, Egypt.
2Molecular Biology, Medical Research Center, Faculty of Medicine, Ain Shams University, Egypt.
3Theodore Bilharz Research Institute, Egypt.
Abstract
Schistosomiasis haematobium is a major endemic parasitic disease in many tropical regions including Egypt. Typical infection results in haematuria, dysuria, anaemia, genital as well as urinary tract lesions, with prospect of kidney damage in complicated cases. In addition, deposited eggs in the tissue, eventually leads to squamous cell carcinoma of urinary bladder in chronically infected individuals. Microscopic detection of excreted ova in urine samples remains the gold standard diagnostic method, in spite of its inherited low sensitivity, inconsistent egg excretion and unreliable results in chronic phase of the disease. Moreover due to pre-requisite for skilled personals and pricey equipment, PCR-based technologies are of limited use especially in low-income endemic countries. So emergence of loop-mediated isothermal DNA amplification (LAMP) seemed a promising technique. Our study evaluated application of LAMP technique in detection of S. haematobium DNA in 69 urine samples of suspected patients for urogenital schistosomiasis, versus conventional urine filtration followed by microscopy ova detection method. Specificity of LAMP was tested using other parasites DNA samples that showed no cross reactivity. Furthermore our results of the calculated diagnostic parameters for sensitivity and specificity for LAMP assay were 100%, with 95% CI (88.78%-100%), and 63.16%, with 95% CI (45.99%-78.19%) respectively, moreover Positive likelihood ratio (LR+) 2.7, and Negative likelihood ratio (LR-) 0.0, which display that LAMP
technique is an up-to-date simple, sensitive, diagnostic important tool that could be employed in clinical diagnosis in poorly equipped facilities, as well as in surveillance of infectious diseases. As authors knowledge, this is the first national report evaluation of LAMP technique as a promising diagnostic tool for urogenital schistosomiasis.
Keywords
Urogenital schistosomiasis; S.haematobium; loop-mediated isothermal DNA amplification (LAMP)
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