DOT-ELISA AS A FIELD TEST FOR HYDATID DIAGNOSIS
ABOU-ELHAKAM, H., FARID, A., MAHANA, N., BAUIOMY, I., ELAMEER, A. (2016). DOT-ELISA AS A FIELD TEST FOR HYDATID DIAGNOSIS. EKB Journal Management System, 46(2), 441-452. doi: 10.21608/jesp.2016.88731
HANY M. ABOU-ELHAKAM; ALYAA A. FARID; NOHA A. MAHANA; IBRAHIUM R. BAUIOMY; AZZA M. ELAMEER. "DOT-ELISA AS A FIELD TEST FOR HYDATID DIAGNOSIS". EKB Journal Management System, 46, 2, 2016, 441-452. doi: 10.21608/jesp.2016.88731
ABOU-ELHAKAM, H., FARID, A., MAHANA, N., BAUIOMY, I., ELAMEER, A. (2016). 'DOT-ELISA AS A FIELD TEST FOR HYDATID DIAGNOSIS', EKB Journal Management System, 46(2), pp. 441-452. doi: 10.21608/jesp.2016.88731
ABOU-ELHAKAM, H., FARID, A., MAHANA, N., BAUIOMY, I., ELAMEER, A. DOT-ELISA AS A FIELD TEST FOR HYDATID DIAGNOSIS. EKB Journal Management System, 2016; 46(2): 441-452. doi: 10.21608/jesp.2016.88731

DOT-ELISA AS A FIELD TEST FOR HYDATID DIAGNOSIS

Journal of the Egyptian Society of Parasitology
Article 21, Volume 46, Issue 2, August 2016, Page 441-452  XML PDF (6.47 MB)
Document Type: Original Article
DOI: 10.21608/jesp.2016.88731
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Authors
HANY M. ABOU-ELHAKAM1; ALYAA A. FARID1; NOHA A. MAHANA1; IBRAHIUM R. BAUIOMY2; AZZA M. ELAMEER1
1Department of Zoology, Faculty of Science, Cairo University, Egypt.
2Department of Parasitology, Theodor Bilharz Research Institute (TBRI), P.O. Box 30 Imbaba, Giza, Egypt.
Abstract
Cystic hydatid disease (Hydatidosis) is one of the most important parasitic zoonoses and remains a public health and economic problem all over the world. Cyst fluid was obtained from hepatic and pulmonary cysts for demonstration of protoscolices and hooklets. Therefore, a standardized and approachable diagnostic tool for the serodiagnosis of CE is still needed. Dot-ELISA is a solid phase diagnostic method for detection of antigen or antibody that is used widely for diagnosis of protozoan and metazoan diseases of human and animals. In the present study, E. granulosus protoscolex antigen was early detected in patient sera using Dot-ELISA, PAb was prepared from anti-rabbit sera and used for coating and as conjugate in Dot-ELISA technique. 48 patients out of 50 were positive to E. spp. with sensitivity and specificity 96% and 94%, respectively. The PPV was 94% and NPV was 90%. Finally, the present results showed that the Dot-ELISA was easy to perform, not expensive, safe, and with good sensitivity and specificity.
Keywords
Egypt; Cystic echinococcosis; Diagnosis; Dot-ELISA; pAb
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