GENETIC POLYMORPHISM OF TOXOPLASMA GONDII FROM RECENTLY INFECTED ABORTED EGYPTIAN WOMEN
BADR, M., ATTIA, S., EL-SHERBINY, W., EL EBIDI, A., HEFNY, H., SALEM, A. (2016). GENETIC POLYMORPHISM OF TOXOPLASMA GONDII FROM RECENTLY INFECTED ABORTED EGYPTIAN WOMEN. EKB Journal Management System, 46(1), 49-55. doi: 10.21608/jesp.2016.88933
MOHAMED S. BADR; SAMAR S. ATTIA; WALID S. EL-SHERBINY; ABD-ALLAH M.A. EL EBIDI; HESHAM M. HEFNY; AHMED N.M. SALEM. "GENETIC POLYMORPHISM OF TOXOPLASMA GONDII FROM RECENTLY INFECTED ABORTED EGYPTIAN WOMEN". EKB Journal Management System, 46, 1, 2016, 49-55. doi: 10.21608/jesp.2016.88933
BADR, M., ATTIA, S., EL-SHERBINY, W., EL EBIDI, A., HEFNY, H., SALEM, A. (2016). 'GENETIC POLYMORPHISM OF TOXOPLASMA GONDII FROM RECENTLY INFECTED ABORTED EGYPTIAN WOMEN', EKB Journal Management System, 46(1), pp. 49-55. doi: 10.21608/jesp.2016.88933
BADR, M., ATTIA, S., EL-SHERBINY, W., EL EBIDI, A., HEFNY, H., SALEM, A. GENETIC POLYMORPHISM OF TOXOPLASMA GONDII FROM RECENTLY INFECTED ABORTED EGYPTIAN WOMEN. EKB Journal Management System, 2016; 46(1): 49-55. doi: 10.21608/jesp.2016.88933

GENETIC POLYMORPHISM OF TOXOPLASMA GONDII FROM RECENTLY INFECTED ABORTED EGYPTIAN WOMEN

Journal of the Egyptian Society of Parasitology
Article 6, Volume 46, Issue 1, April 2016, Page 49-55  XML PDF (3.61 MB)
Document Type: Original Article
DOI: 10.21608/jesp.2016.88933
View on SCiNiTO View on SCiNiTO
Authors
MOHAMED S. BADR1; SAMAR S. ATTIA2; WALID S. EL-SHERBINY3; ABD-ALLAH M.A. EL EBIDI4; HESHAM M. HEFNY5; AHMED N.M. SALEM5
1Department of Molecular Biology, Medical Research Center, Faculty of Medicine, Ain Shams University, Egypt.
2Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt.
3Department of Obstetrics & Gynaecology, Faculty of Medicine, Cairo University, Egypt.
4Department of Medical Biochemistry, Faculty of Medicine, Aswan University, Egypt.
5Department of Clinical Pathology, Faculty of Medicine, Sohag University, Egypt.
Abstract
Toxoplasmosis caused by Toxoplasma gondii is one of the most prevalent parasitic diseases in human beings. Human toxoplasmosis can be associated with serious clinical manifestations, particularly in developing fetus. The aim of the current study was to identify the possible lineage type of Toxoplasma gondii, molecularly detected in placental samples of women whose pregnancies were spontaneously terminated in the first trimester. Preliminary detection of Toxoplasma
genomic materials was done by a SYBR green qPCR technology. Subsequent identification of Toxoplasma strain was done for the positive samples using PCR- restriction fragment length polymorphism (RFLP) at the SAG2 loci of T. gondii using restriction enzymes HhaI and Sau3AI. Out of 72 tested samples, Toxoplasma B1 gene was detected in 9 cases. Toxoplasma genotypes I and II in addition to unknown type were identified in 4, 3 and 2 cases respectively, while type III
was not detected in our samples, hence excluded as a leading cause of abortion in humans in our preliminary study. Nevertheless, it remains uncertain to what extent the genotype of the parasite directly contributes to the clinical severity of human toxoplasmosis. Certainly, advanced molecular techniques targeting different Toxoplasma strains are crucial for better understanding of human toxoplasmosis. For more elucidation, additional studies are recommended intended for genetic characterization of such serious parasitic infection using larger number of samples.
Keywords
abortion; genotype; RFLP; Toxoplasma gondii
Statistics
Article View: 157
PDF Download: 184