COMPARATIVE STUDY ON IMMUNOBLOT VERSUS PCR IN DIAGNOSIS OF SCHISTOSOMIASIS MANSONI IN EXPERIMENTAL INFECTED MICE | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Article 11, Volume 46, Issue 1, April 2016, Page 101-108 PDF (3.21 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jesp.2016.88954 | ||||
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| Authors | ||||
| MOUSA A.M. ISMAIL1; WAHED MOHAMMED ALI MOUSA2; ENAS YAHIA ABU-SAREA3; MAHA M. A. BASYOUNI1; SAMAH SAYED MOHAMMED3 | ||||
| 1Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt. | ||||
| 2Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Egypt. | ||||
| 3Department of Medical Parasitology, Faculty of Medicine, Beni-Suef University, Egypt. | ||||
| Abstract | ||||
| This study compared PCR and Western blot techniques in diagnosis of schistosomiasis mansoni. Forty Swiss albino mice were used, thirty two mice were infected with cercariae of S. mansoni and eight mice were kept uninfected which were used as a control. Blood was obtained from four infected mice weekly beginning from the 1st week to the 8th week post infection. The study found that PCR was positive from the first week post infection, while Western blot technique was positive from the second week post infection. Thus, PCR diagnosed schistosomiasis mansoni earlier than Western blot technique, but both were able to diagnose. | ||||
| Keywords | ||||
| Egypt; Schistosoma mansoni; PCR; Western blot technique | ||||
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