LYMPHO-PROLIFERATIVE RESPONSES TO VARIOUS FASCIOLA HEPATICA WORM'S ANTIGENS: AN IN VITRO STUDY
SHARAF, O., AMIR, E., HAWASH, Y. (2016). LYMPHO-PROLIFERATIVE RESPONSES TO VARIOUS FASCIOLA HEPATICA WORM'S ANTIGENS: AN IN VITRO STUDY. EKB Journal Management System, 46(1), 217-222. doi: 10.21608/jesp.2016.88976
OSAMA F. SHARAF; ELAMIR M. AMIR; YOUSRY A. HAWASH. "LYMPHO-PROLIFERATIVE RESPONSES TO VARIOUS FASCIOLA HEPATICA WORM'S ANTIGENS: AN IN VITRO STUDY". EKB Journal Management System, 46, 1, 2016, 217-222. doi: 10.21608/jesp.2016.88976
SHARAF, O., AMIR, E., HAWASH, Y. (2016). 'LYMPHO-PROLIFERATIVE RESPONSES TO VARIOUS FASCIOLA HEPATICA WORM'S ANTIGENS: AN IN VITRO STUDY', EKB Journal Management System, 46(1), pp. 217-222. doi: 10.21608/jesp.2016.88976
SHARAF, O., AMIR, E., HAWASH, Y. LYMPHO-PROLIFERATIVE RESPONSES TO VARIOUS FASCIOLA HEPATICA WORM'S ANTIGENS: AN IN VITRO STUDY. EKB Journal Management System, 2016; 46(1): 217-222. doi: 10.21608/jesp.2016.88976

LYMPHO-PROLIFERATIVE RESPONSES TO VARIOUS FASCIOLA HEPATICA WORM'S ANTIGENS: AN IN VITRO STUDY

Journal of the Egyptian Society of Parasitology
Article 24, Volume 46, Issue 1, April 2016, Page 217-222  XML PDF (2.81 MB)
Document Type: Original Article
DOI: 10.21608/jesp.2016.88976
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Authors
OSAMA F. SHARAF1; ELAMIR M. AMIR2, 3; YOUSRY A. HAWASH2, 4
1Department of Medical Parasitology, National Liver Institute, Menoufia University, Egypt.
2Department of Medical Parasitology, National Liver Institute, Menoufia University, Egypt.+
3Department of Medical Parasitology, Rabigh Faculty of Medicine, King AbdulAziz University, KSA.
4Department of Medical Parasitology, Taif Faculty of Medicine, Taif University, KSA.
Abstract
Fascioliasis is an important zoonotic disease with approximately 2-4 million people infected worldwide and a further 180 million at risk of infection. F. hepatica can survive within the bile ducts for many years through its ability to suppress the host immunity with Fasciola cathepsin L1 cysteine protease and Glutathione S transferase playing an important role. The aim of the present study is to investigate the in vitro lympho-proliferative responses of hepatic hilar lymphocytes (HLN) of infected sheep in response to different F. hepatica antigens. The suppressive effects of Fasciola excretory/secretory (ES) and tegument (TEG) and their fractions were also investigated. Our results showed that both ES and TEG had significant suppressive effects on lymphoproliferation, up to 74% and 92%, respectively. When these antigens were fractionated, fraction 3 (MW of ˃10000-30000) of both ES (64%) and TEG (59%) in addition to fraction 4 (MW of ≤ 10000) of TEG (38%) inherited the suppressive effects. Identification of the potential molecule(s) with such suppressive effects on lymphocytes in TEG fraction 4 could reveal vaccine candidates.
Keywords
Fasciola hepatica; tegument; excretory/secretory; lympho-proliferation
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