DETECTION OF Brucella melitensis FROM FILTERED BOVINE PERIPHERAL-BLOOD BY POLYMERASE CHAIN REACTION TEST | ||||
Journal of Agricultural Chemistry and Biotechnology | ||||
Article 3, Volume 1, Issue 4, April 2010, Page 229-234 PDF (347.8 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jacb.2010.89424 | ||||
View on SCiNiTO | ||||
Authors | ||||
Buthaina Al-Ashkar1; A. Al-Mariri2; Ibtissam Hamad3 | ||||
1Dept. of Plant Biology, Faculty of Science, University of Damascus | ||||
2Dept. of Molecular Biology and Biotechnology, Atomic Energy Commission, Damascus | ||||
3Dept. of Environment, Faculty of Science, University of Damascus | ||||
Abstract | ||||
Brucella melitensis contaminants of bovine peripheral-blood can be concentrated and isolated by filtration; and their DNA can be released for subsequent PCR detection by heating the isolated colony at 95°C for 10 min. Detecting rate of brucellosis in the infected sample was less than 20 CFU/ml of blood, after leukocytes filtration and culturing, as well as on isolated colonies. This PCR method also enables us to make the diagnosing of brucellosis for the animals with a negative complement fixation test. | ||||
Keywords | ||||
Blood; Brucella melitensis; Leukocytes; PCR | ||||
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