Management of Poinsettia Leaf Blight caused by Botrytis cinerea using Fenton-based Solutions | ||||
Egyptian Journal of Phytopathology | ||||
Article 4, Volume 45, Issue 1, June 2017, Page 45-56 PDF (473.07 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejp.2017.89526 | ||||
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Authors | ||||
Nour El-Houda Reyad* 1; Naglaa T. Mohamed 2 | ||||
1Plant Pathol. Dept., Fac. Agric., Cairo Univ., Egypt | ||||
2Plant Pathology Research Institute, Agricultural Research Center, 12619, Giza, Egypt | ||||
Abstract | ||||
The present work was carried out to determine the effect of certain Fenton-based solutions, i.e. Fenton, Fenton like and Fenton oxalate complex compared to chlorothalonile fungicide on poinsettia leaf blight. Botrytis cinerea was isolated and identified morphologically and confirmed at the molecular base using specific primer pairs (C729+) and (C729-). DNA fragment of 700 bp was detected in the genome compared to the control. Moreover, spraying plants with the tested solutions 24hr after inoculation with the pathogen and two times later every seven days in the greenhouse decreased the disease severity. Chlorothalonile followed by Fenton oxalate complex were more effective than Fenton like and Fenton, being 14.45, 24.44, 30.0 and 38.89%, respectively compared to the control showing 54.44%. Furthermore, increasing in some plant growth parameters (Plant height “cm”), number of branches/plant,number of bracts/plant, number of leaves/plant, fresh weight of bracts (g) and fresh weight of leaves (g) and total chlorophyll, as well as oxidative reductive enzymes showed that Fenton oxalate complex was superior in these regards. In conclusion, Fenton based solutions such as Fenton oxalate complex may be used as an alternative to fungicides to manage the poinsettia leaf blight caused by B. cinerea. | ||||
Keywords | ||||
Botrytis cinerea; fenton based solutions; leaf blight; molecular identification; morphological identification and oxidative enzymes | ||||
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