STUDIES ON THE ROLE OF TUMOR NECROSIS FACTOR- ALPHA (TNF-α) IN HEPATOCYTES INDUCED APOPTOSIS IN VACCINATED, SCHISTOSOMA MANSONI-CHALLENGED MICE
ETEWA, S., ABD EL-AAL, N., ABDELRAHMAN, S., ABD EL BARY, E., EL-SHAFEI, M. (2015). STUDIES ON THE ROLE OF TUMOR NECROSIS FACTOR- ALPHA (TNF-α) IN HEPATOCYTES INDUCED APOPTOSIS IN VACCINATED, SCHISTOSOMA MANSONI-CHALLENGED MICE. EKB Journal Management System, 45(1), 47-60. doi: 10.21608/jesp.2015.89686
SAMIA E. ETEWA; NAGLAA F. ABD EL-AAL; SARA A. ABDELRAHMAN; EMAN H. ABD EL BARY; MAHMOUD A. EL-SHAFEI. "STUDIES ON THE ROLE OF TUMOR NECROSIS FACTOR- ALPHA (TNF-α) IN HEPATOCYTES INDUCED APOPTOSIS IN VACCINATED, SCHISTOSOMA MANSONI-CHALLENGED MICE". EKB Journal Management System, 45, 1, 2015, 47-60. doi: 10.21608/jesp.2015.89686
ETEWA, S., ABD EL-AAL, N., ABDELRAHMAN, S., ABD EL BARY, E., EL-SHAFEI, M. (2015). 'STUDIES ON THE ROLE OF TUMOR NECROSIS FACTOR- ALPHA (TNF-α) IN HEPATOCYTES INDUCED APOPTOSIS IN VACCINATED, SCHISTOSOMA MANSONI-CHALLENGED MICE', EKB Journal Management System, 45(1), pp. 47-60. doi: 10.21608/jesp.2015.89686
ETEWA, S., ABD EL-AAL, N., ABDELRAHMAN, S., ABD EL BARY, E., EL-SHAFEI, M. STUDIES ON THE ROLE OF TUMOR NECROSIS FACTOR- ALPHA (TNF-α) IN HEPATOCYTES INDUCED APOPTOSIS IN VACCINATED, SCHISTOSOMA MANSONI-CHALLENGED MICE. EKB Journal Management System, 2015; 45(1): 47-60. doi: 10.21608/jesp.2015.89686

STUDIES ON THE ROLE OF TUMOR NECROSIS FACTOR- ALPHA (TNF-α) IN HEPATOCYTES INDUCED APOPTOSIS IN VACCINATED, SCHISTOSOMA MANSONI-CHALLENGED MICE

Journal of the Egyptian Society of Parasitology
Article 7, Volume 45, Issue 1, April 2015, Page 47-60  XML PDF (525.3 K)
Document Type: Original Article
DOI: 10.21608/jesp.2015.89686
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Authors
SAMIA E. ETEWA1; NAGLAA F. ABD EL-AAL1; SARA A. ABDELRAHMAN1; EMAN H. ABD EL BARY2; MAHMOUD A. EL-SHAFEI3
1Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
2Department of Pathology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
3Department of Clinical Pathology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Abstract
Tumour Necrosis Factor-alpha (TNF-α) plays a complex role in pathophysiological changes caused by schistosomiasis in the liver cells as induced apoptosis. So, The highlighted experimentally the role of TNF-α in hepatocytes apoptosis, using that as an assessment of the efficacy of antischistosomal vaccination by mixed crude antigens preparations [Cercarial antigen preparation (CAP) + soluble worm antigen preparation (SWAP) + soluble egg antigen(SEA)] by parasitological, histo-pathological and histochemical studies using Feulgen stain of hepatoytes DNA, a serological study also of serum TNF- α level by ELISA. Fifty two laboratory bred Albino male mice, were used in this study. They were classified into four groups (13 mice in each group), G1: normal control, G2 as infected control while G3 supported by Freund's Adjuvant (F.
Adj) then infected and G4 vaccinated with combined antigens (CAP, SWAP and SEA) + F. Adj, then infected. Mice were sacrificed by cervical dislocation 9 weeks post infection, parasitological (Kato-Katz thick smear for egg count), histopathologial {haematoxylin and eosin (H&E) staining of hepatic sections}, histochemical (feulgen staining of hepatocytes DNA) and ELISA to estimate serum TNF-α level were performed. The data showed that vaccination with combined antigens showed protective effect on vaccinated then Schistosoma challenged mice, hepatocytes induced apoptosis was directly proportional with the TNF-α serum level, and the protection degree of potential combined vaccine was inversely proportional with serum TNF-α level and induced apoptosis.
Keywords
S. mansoni; TNF-α; CAP; SWAP; SEA; feulgen stain; apoptosis
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