SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS (SDS-PAGE) FOR DIFFERENTIATION OF DIFFERENT SCHISTOSOME AND FASCIOLID SPECIES
ELSAGHEIR, A., ABD ELLAH, O., SAYED, F., MONIB, M., ABDEL-KADER, M. (2015). SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS (SDS-PAGE) FOR DIFFERENTIATION OF DIFFERENT SCHISTOSOME AND FASCIOLID SPECIES. EKB Journal Management System, 45(2), 325-330. doi: 10.21608/jesp.2015.89911
ASMAA M.A. ELSAGHEIR; OSAMA H. ABD ELLAH; FATMA G. SAYED; MOHAMMED E.M. MONIB; MOHAMMAD S. ABDEL-KADER. "SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS (SDS-PAGE) FOR DIFFERENTIATION OF DIFFERENT SCHISTOSOME AND FASCIOLID SPECIES". EKB Journal Management System, 45, 2, 2015, 325-330. doi: 10.21608/jesp.2015.89911
ELSAGHEIR, A., ABD ELLAH, O., SAYED, F., MONIB, M., ABDEL-KADER, M. (2015). 'SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS (SDS-PAGE) FOR DIFFERENTIATION OF DIFFERENT SCHISTOSOME AND FASCIOLID SPECIES', EKB Journal Management System, 45(2), pp. 325-330. doi: 10.21608/jesp.2015.89911
ELSAGHEIR, A., ABD ELLAH, O., SAYED, F., MONIB, M., ABDEL-KADER, M. SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS (SDS-PAGE) FOR DIFFERENTIATION OF DIFFERENT SCHISTOSOME AND FASCIOLID SPECIES. EKB Journal Management System, 2015; 45(2): 325-330. doi: 10.21608/jesp.2015.89911

SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS (SDS-PAGE) FOR DIFFERENTIATION OF DIFFERENT SCHISTOSOME AND FASCIOLID SPECIES

Journal of the Egyptian Society of Parasitology
Article 12, Volume 45, Issue 2, August 2015, Page 325-330  XML PDF (2.63 MB)
Document Type: Original Article
DOI: 10.21608/jesp.2015.89911
View on SCiNiTO View on SCiNiTO
Authors
ASMAA M.A. ELSAGHEIR1; OSAMA H. ABD ELLAH1; FATMA G. SAYED2; MOHAMMED E.M. MONIB2; MOHAMMAD S. ABDEL-KADER3
1Department of Medical Parasitology, Faculty of Medicine, South Valley University, Egypt.
2Department of Medical Parasitology, Faculty of Medicine, Assuit University, Egypt.
3Department of Urology, Faculty of Medicine, South Valley University, Egypt.
Abstract
Fasciola and Schistosoma spp. are digenetic trematodes that have a major detrimental impact on human health worldwide. It is not unusual to find common molecules among parasites of different species, genera, or phyla. In this study Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was used to identify the common proteins of adult Fasciola and Schistosoma spp. Adult Fasciola spp, were collected from the bile ducts of naturally infected
cattle and sheep. Schistosoma mansoni and S. haematobium adults surface antigens were prepared in Theodor Bilharz Research Institute were used. Results showed sharing bands between all worms, which have the same molecular weight of 36 kDa. On the other hand, band with molecular weight 41 kDa was sharing between them except F. hepatica. Bands at 48 and 170 kDa were sharing between all species of Fasciola spp and Schistosoma mansoni.
Keywords
F. gigantica; F. hepatica; S. mansoni; S. haematobium; electrophoresis; Bands
Statistics
Article View: 96
PDF Download: 129