MICROPROPAGATION OF DATE PALM CV. MALAKABY THROUGH SOMATIC EMBRYOGENESIS 2- EFFECT OF ADENINE HEMISULFATE, GLUTAMINE AND GLUTATHIONE | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 7, Volume 34, Issue 3, March 2009, Page 1613-1628 PDF (879.71 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2009.90292 | ||||
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| Authors | ||||
| A. Hegazy; M. I. Nasr; I. A. Ibrahim; H. H. El-Bastawissy | ||||
| Genetic Engineering and Biotechnology Research Institute (GEBRI), Plant Biotech. Department., Minufiya University, Sadat City, Egypt | ||||
| Abstract | ||||
| Addition of chemical compounds (adenine hemisulfate glutamine or glutathione) to the culture medium of date palm (Phoenix dactylifera. L.) dry cultivar, i.e. Malakaby affected positively repetitive somatic embryos cycle, plantlets formation and subsequently acclimatization as compared with the control. Repetitive somatic embryos cultured on modified MS medium (MMS) supplemented with NAA 0.1 mg/l, Kin 0.5 mg/l and activated charcoal 1.5 g/l in addition to adenine hemisulfate at the concentration of 40 mg/l and solidified with gelrite 1.5 g/l recorded the highest no. of embryos, multiplication rate, fresh weight and growth value after 3 weeks of culture incubation. However, glutamine at the concentration of 200 mg/l recorded higher embryos multiplication rate, fresh weight and growth value. On the other hand, glutathione at the concentration of 15 mg/l recorded higher significant values of no. of embryos and embryos multiplication rate as well as higher fresh weight and growth value. Resulted shootlets were cultured individually on MS basal medium supplemented with NAA 0.5 mg/l and IBA 0.5 mg/l in addition to the same chemical compounds concentrations previously tested and solidified with 6 g/l phyto agar. Well- rooted plantlets obtained were transferred ex vitro for acclimatization on soil type mixture of compost and perlite (1:1 v/v). Addition of adenine hemisulfate (40 mg/l) or glutamine (100 mg/l) to the rooting medium recorded the highest growth value and no. of leaves respectively as well as the highest no. of roots after 8 weeks of culture incubation and raised the same values of plantlets survival (13.33 %) over the control after 3 months in acclimatization. However, addition of glutathione at the concentration of 15 mg/l to the rooting medium recorded higher values of no. of leaves, fresh weight, no. of roots and raised plantlets survival (20 %) over the control in acclimatization. | ||||
| Keywords | ||||
| Phoenix dactylifera L; in vitro; embryogenesis; callus; chemical compounds | ||||
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