MICROPROPAGATION OF DATE PALM CV. MALAKABY THROUGH SOMATIC EMBRYOGENESIS 3- EFFECT OF TRYPTONE, YEAST EXTRACT, CASEIN HYDROLYSATE AND PINEAPPLE EXTRACT | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 8, Volume 34, Issue 3, March 2009, Page 1629-1644 PDF (712.66 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2009.90293 | ||||
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| Authors | ||||
| A. Hegazy; M. I. Nasr; I. A. Ibrahim; H. H. El-Bastawissy | ||||
| Genetic Engineering and Biotechnology Research Institute (GEBRI), Plant Biotech. Deparment., Minufiya University, Sadat City, Egypt | ||||
| Abstract | ||||
| Date palm (Phoenix dactylifera. L.) dry cultivar, i.e. Malakaby repetitive somatic embryos clusters cultured on MS (Murashige and Skooge, 1962) modified medium (MMS) supplemented with NAA (0.1 mg/l), kin (0.5), activated charcoal (1.5 g/l) and gelrite (1.5 g/l) in addition to tryptone at the concentration of 1.0 g/l recorded higher significant number of embryos and multiplication rate, also higher fresh weight and growth value. However, addition of yeast extract at the concentration of 1.5 g/l recorded higher significant number of embryos as well as higher embryos multiplication rate, fresh weight and growth value. Also, addition of casein hydrolysate at the concentration of 1.5 g/l recorded higher significant number of embryos as well as higher embryos multiplication rate, fresh weight and growth value. While, addition of pineapple extract at the concentration of 9 g/l recorded higher significant value of all growth characters as compared with the control. Resulted shootlets were cultured individually on MS basal medium supplemented with NAA 0.5 mg/l and IBA 0.5 mg/l in addition to the same chemical compounds concentrations previously tested and solidified with 6 g/l phyto agar. Well- rooted plantlets obtained were transferred ex vitro for acclimatization on soil type mixture of compost and perlite (1:1 v/v). Addition of tryptone at the concentration i.e. 1, 1.5 and 2 g/l to the rooting medium decreased all morphological characters after 8 weeks of incubation and declined plantlets survival (26.67, 13.33 and 6.67 %) respectively compared to the control (40 %) after 3 months in acclimatization. However, addition of yeast extract at the concentration of 1.5 g/l to the rooting medium recorded higher no. of leaves, leaf length, no. of roots and raised survival in acclimatization (26.67 %) over the control. While, addition of casein hydrolysate at the concentration of 1.5 g/l to the rooting medium recorded higher significant values of number of roots and raised survival in acclimatization (13.33 %). On the other hand, addition of pineapple extract at the concentration of 9 g/l to the rooting medium recorded higher values of all growth characters and raised plantlets survival in acclimatization (33.33 %) over the control. | ||||
| Keywords | ||||
| Phoenix dactylifera L; in vitro; embryogenesis; callus; complex addenda | ||||
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