Isolation and identification of Cronobacter species from some animal sources | ||||
Benha Veterinary Medical Journal | ||||
Article 23, Volume 37, Issue 1, September 2019, Page 112-117 PDF (827.77 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/bvmj.2019.17855.1106 | ||||
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Authors | ||||
Ashraf Abd El Tawab1; Amira Mohamed 2; Ahmed Ammar3; Mohamed Mohamed4 | ||||
1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University | ||||
2Faculty of Veterinary Medicine, Benha Univ., Egypt | ||||
3Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University | ||||
4Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University | ||||
Abstract | ||||
This work was carried out to study the bacteriological importance of Cronobacter sakazakii as a potential foodborne emerging pathogen involved in severe illness and deaths in humans, especially neonates due to consumption of contaminated infant powdered infant formula (milk and food). A total of 100 samples [PIF milk (n=55), PIF food (n=15), milk powder (n=15) and milk powder products (n=15) were collected and subjected to bacteriological examination for the presence of Cronobacter. Six samples out of 100 examined (6%) were found positive for Cronobacter spp. The isolation rates were 4% in PIF milk and 1% in PIF food. The identity of the isolated organism was confirmed as Cronobacter spp. by subjecting the bacteriologically positive samples to PCR technique using 16S rRNA species specific primers. Cronobacter specific 16S rRNA was detected respectively in 3/5 and 1/5 of bacteriologically positive PIF milk and PIF food examined. All positive 16S rRNA (n=4) were examined for the presence of C. sakazakii. C. sakazakii were confirmed in an isolate from 3 isolates of PIF milk and one of PIF food. The outer membrane protein A (ompA) gene was detected in 2 identified C. sakazakii isolates, while gene encoding for zinc-metaloprotease (zpx) was only identified in 3 C. sakazakii isolates | ||||
Keywords | ||||
Cronobacter; animal; Bacteria; Milk powder | ||||
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