Seroprevalence and molecular detection of isolated BoHV-1 among farm animals
Elashmawy, A., Ali, S., El-Habbaa, A., Bastawecy, I. (2019). Seroprevalence and molecular detection of isolated BoHV-1 among farm animals. EKB Journal Management System, 37(1), 137-143. doi: 10.21608/bvmj.2019.17291.1098
Asmaa Mounier Elashmawy; Saad Sharawi Ali; ayman said El-Habbaa; Iman Mohamed Bastawecy. "Seroprevalence and molecular detection of isolated BoHV-1 among farm animals". EKB Journal Management System, 37, 1, 2019, 137-143. doi: 10.21608/bvmj.2019.17291.1098
Elashmawy, A., Ali, S., El-Habbaa, A., Bastawecy, I. (2019). 'Seroprevalence and molecular detection of isolated BoHV-1 among farm animals', EKB Journal Management System, 37(1), pp. 137-143. doi: 10.21608/bvmj.2019.17291.1098
Elashmawy, A., Ali, S., El-Habbaa, A., Bastawecy, I. Seroprevalence and molecular detection of isolated BoHV-1 among farm animals. EKB Journal Management System, 2019; 37(1): 137-143. doi: 10.21608/bvmj.2019.17291.1098

Seroprevalence and molecular detection of isolated BoHV-1 among farm animals

Benha Veterinary Medical Journal
Article 28, Volume 37, Issue 1, September 2019, Page 137-143  XML PDF (988.47 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2019.17291.1098
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Authors
Asmaa Mounier Elashmawy email 1; Saad Sharawi Aliorcid 2; ayman said El-Habbaaorcid 3; Iman Mohamed Bastawecy4
1virology department, animal health institute
2Department of virology , faculty of veterinary medicine, benha university
3Department of Virology, Faculty of Veterinary Medicine, Benha University
4Virology Department, Animal Health Research Institute, Dokki, Giza, Egypt
Abstract
Bovine herpes virus type 1 (BoHV-1) is an economically important, worldwide distributed pathogen of domestic and wild Bovidae. It causes respiratory tract manifestations and/or abortion.The present study was achieved to study seroprevalence and make isolation of BoHV-1 from suspected cattle,buffaloes,sheep and goats collected from three governorates in Egypt (Menofya, Kalubeya and Dakahlia) through the years 2017- 2018.Serum and milk samples obtained from apparently healthy non-vaccinated animals were tested using indirect antibody detection ELISA. A total of 78 serum samples out of 346 samples and 46 milk samples out of 300 samples were positive. Virus was isolated from nasal swabs and lung tissue samples onchorioallantoic membrane (CAM) of 11-day-old embryonated chicken eggs. It showed congestion, edematous vacuoles and thickening small foci ranged from 2 to 3 mm in diameter, scattered on CAM membrane.Examination by electron microscope using positive staining methods showed the characteristic morphology of the herpes virion. The isolated virus was also genotypically confirmed to be BoHV-1 in a PCR assay using primers which specifically amplified a fragment (575 bp) of gC encoding gene in the viral DNA genome
Keywords
BoHV-1; seroprevalence; ECE; PCR
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