Prevalence and molecular characterization of Lumpy Skin Disease in cattle during period 2016-2017 | ||||
Benha Veterinary Medical Journal | ||||
Article 35, Volume 37, Issue 1, September 2019, Page 172-175 PDF (833 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/bvmj.2019.18293.1118 | ||||
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Authors | ||||
Mona Abd Elmohsen ![]() ![]() | ||||
1Faculty of Vet. Med., Benha University, Egypt. | ||||
2Department of Animal Medicine, Faculty of Veterinary Medicine, Benha University | ||||
3Faculty of Veterinary medicine Benha University | ||||
Abstract | ||||
The present study was carried out to determine the prevalence of Lumpy skin disease (LSD) in different governorates in Egypt in relation to different factors. A total of 875 blood samples and 10 specimens (skin nodules) were collected from apparently health and clinically infected balady cattle of different ages and two sexes in different seasons during period 2016 – 2017. The prevalence of LSD in cattle in different governorates was 35.4%, 29.6%, 25.3%, 24.7%, 15.6% and 13.8% in Beni Suef, El-Faiyum, EL-Dakahlia, El-Qalyubia, El-Beheira and El-Gharbia, respectively. The prevalence of the disease was higher in females (29.2%) than males (21.2%). Also, the prevalence rate was higher in age group (> 12-36 m) 33 % and (> 36-50 m) 23.5% in comparison with age groups (3-6 m) 15.6% and (> 6-12 m) 16%. The disease was highly occurred during summer and autumn (27.6% and 26%, respectively). PCR reaction was carried out on skin nodules samples to confirm and characterize LSDV. selective positive samples undergo sequencing and compared with other LSDV strains and also other Capri-poxviruses in database in GenBank. The local LSDV samples reveled high identity in phylogenetic analysis to each other and high identity and clustered with other world LSDV strains and Capri-poxvirust. | ||||
Keywords | ||||
LSD; ELISA; PCR; sequencing; phylogenetic | ||||
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