The potential Anti-Inflammatory and antioxidant activities of Proanthocyanidins in acetic acid–induced ulcerative colitis in rats
Hussein, S., Abou Zaid, O., Abdel Latif, S., EL-Sebaey, A. (2019). The potential Anti-Inflammatory and antioxidant activities of Proanthocyanidins in acetic acid–induced ulcerative colitis in rats. EKB Journal Management System, 37(1), 245-252. doi: 10.21608/bvmj.2019.14356.1036
Samy Ali Hussein; Omayma A.R. Abou Zaid; Samir Abdel Latif; Anhar Mohamed EL-Sebaey. "The potential Anti-Inflammatory and antioxidant activities of Proanthocyanidins in acetic acid–induced ulcerative colitis in rats". EKB Journal Management System, 37, 1, 2019, 245-252. doi: 10.21608/bvmj.2019.14356.1036
Hussein, S., Abou Zaid, O., Abdel Latif, S., EL-Sebaey, A. (2019). 'The potential Anti-Inflammatory and antioxidant activities of Proanthocyanidins in acetic acid–induced ulcerative colitis in rats', EKB Journal Management System, 37(1), pp. 245-252. doi: 10.21608/bvmj.2019.14356.1036
Hussein, S., Abou Zaid, O., Abdel Latif, S., EL-Sebaey, A. The potential Anti-Inflammatory and antioxidant activities of Proanthocyanidins in acetic acid–induced ulcerative colitis in rats. EKB Journal Management System, 2019; 37(1): 245-252. doi: 10.21608/bvmj.2019.14356.1036

The potential Anti-Inflammatory and antioxidant activities of Proanthocyanidins in acetic acid–induced ulcerative colitis in rats

Benha Veterinary Medical Journal
Article 48, Volume 37, Issue 1, September 2019, Page 245-252  XML PDF (973.14 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2019.14356.1036
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Authors
Samy Ali Hussein1; Omayma A.R. Abou Zaid2; Samir Abdel Latif3; Anhar Mohamed EL-Sebaey email 4
1Department of Biochemistry, Faculty of Vet. Med., Benha University, Egypt.
2Clinical Biochemistry Department, Faculty of Veterinary Medicine, Benha University, Egypt
3Department of Animal Hygiene, Behavior and Management, Faculty of Veterinary Medicine, Benha University, Egypt
4Biochemistry,veterinary,benha,benha,egypt
Abstract
Objective: The present study was designed to investigate the anti-colitic effect of Proanthocyanidins (Pcs) on acetic acid -induced ulcerative colitis in rats. Methods: Forty male rats (220-250 g) were divided into five equal groups. Group Ι:(Control normal group) rats received no drugs. Group Π: (Early UC-induced group) rats received 3% acetic acid (2ml/rat) intracolonially and sacrificed 3 days later from induction. Group III:(Late UC –induced group) rats received acetic acid similar to group 2 and sacrificed after 21 days. Group IV:(Early UC+Pcs protected group) rats received Pcs (200 mg/kg body weight/day) orally for 21 days prior to acetic acid administration for UC induction. Group V:(Late UC+Pcs treated group) treated with Pcs as in group III for 21 days after UC induction. Results: A significant increase in L-MDA and MOP with marked decrease in GSH and CAT activity in colon of acetic acid -induced colitis in rats as compared with control group. However, a significant depletion of colon tissue L-MDA, MOP and marked increase in GSH and CAT activity were observed after Pcs treatment compared to ulcerated untreated rats. The qPCR results revealed a significant upregulation of mRNA gene expression levels of TNF-α, COX-2 and a significant downregulation in TGF-β1 in colon of acetic acid -induced UC in rats. The expression levels of TNF-α, COX-2 was significantly downregulated and a significant upregulated in TGF-β1 in colon tissues after administration of Pcs. Conclusions: Proanthocyanidins protects rats colon mucosa damage against acetic acid -induced ulcerative colitis via anti-inflammatory and anti-oxidative mechanisms.
Keywords
Proanthocyanidins; acetic acid; Colitis; anti-inflammatory; transforming growth factor
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