Biological and Molecular Variability of Rice Sheath Rot Pathogen Sarocladium oryzae using SCAR and SRAP Markers | ||||
Egyptian Journal of Phytopathology | ||||
Article 12, Volume 44, Issue 1, June 2016, Page 157-173 PDF (549.24 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejp.2016.92374 | ||||
View on SCiNiTO | ||||
Authors | ||||
Mona M. Saleh 1; Ismail Khatab2; Rabie El-Shafey3 | ||||
1Plant Pathology Research Institute, Agricultural Research Center, 12619, Giza, Egypt. | ||||
2Genetics Dept., Fac. Agric., Kafer El-Sheikh Univ., Egypt | ||||
3Rice Res. Training Centre, Field Crops Res. Inst., ARC, Egypt | ||||
Abstract | ||||
Rice sheath rot disease caused by Sarocladium oryzae is a seed borne disease and is a major production constraint in some rice growing countries. This study aimed to evaluate some of our local rice entries to sheath rot infection with different isolates, elucidate their phytotoxicity effect and the molecular variability among some selected isolates derived from different locations. Data revealed that Sakha 101 was the most resistant cultivar against sheath rot. The Toxin of S. oryzae completely inhibited differentiation of rice roots and negatively affected seed germination of rice and weeds. S. oryzae isolates showed morphological variability and polymorphism in DNA, high levels of genetic variability among isolates were identified. Using MR specific primers revealed that the two bands (1500 and 1400bp) were found only in low virulent isolates. Using SRAP dendogram supported the similarity of data. Moreover, using SCAR and SRAP primers could be able to differentiate among S. oryzae isolates collected from different locations or varieties and differed in their virulence and classified in two main clusters as high and low virulent. Thus, the molecular variability of S. oryzae isolates may be an important consideration in breeding programs to develop resistance for sheath rot disease. | ||||
Keywords | ||||
Breeding programs; molecular variability; rice entries; Sarocladium oryzae and sheath rot | ||||
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