Identification of emerging Acinetobacter johnsonii virulence and antibiotic resistance genes associated with high mortality in cultured Oreochromis niloticus
Elabd, H., abd el-latif, A., Shaheen, A., matter, A. (2020). Identification of emerging Acinetobacter johnsonii virulence and antibiotic resistance genes associated with high mortality in cultured Oreochromis niloticus. EKB Journal Management System, 10(2), 19-33. doi: 10.21608/eja.2020.29010.1021
Hiam Elabd; ashraf abd el-latif; Adel Shaheen; Aya matter. "Identification of emerging Acinetobacter johnsonii virulence and antibiotic resistance genes associated with high mortality in cultured Oreochromis niloticus". EKB Journal Management System, 10, 2, 2020, 19-33. doi: 10.21608/eja.2020.29010.1021
Elabd, H., abd el-latif, A., Shaheen, A., matter, A. (2020). 'Identification of emerging Acinetobacter johnsonii virulence and antibiotic resistance genes associated with high mortality in cultured Oreochromis niloticus', EKB Journal Management System, 10(2), pp. 19-33. doi: 10.21608/eja.2020.29010.1021
Elabd, H., abd el-latif, A., Shaheen, A., matter, A. Identification of emerging Acinetobacter johnsonii virulence and antibiotic resistance genes associated with high mortality in cultured Oreochromis niloticus. EKB Journal Management System, 2020; 10(2): 19-33. doi: 10.21608/eja.2020.29010.1021

Identification of emerging Acinetobacter johnsonii virulence and antibiotic resistance genes associated with high mortality in cultured Oreochromis niloticus

Egyptian Journal for Aquaculture
Article 3, Volume 10, Issue 2, June 2020, Page 19-33  XML PDF (528.85 K)
Document Type: Research article
DOI: 10.21608/eja.2020.29010.1021
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Authors
Hiam Elabdorcid 1; ashraf abd el-latif email orcid 2; Adel Shaheen3; Aya matter4
1Department of Aquatic animals Diseases and Management, Faculty of Veterinary Medicine, Benha University,
2Benha Univ. Fac. of vet.Med. Department of aquatic animals diseases
3Faculty of Veterinary, Benha University, Egypt
4Department of Aquatic animals Diseases and Management, Faculty of Veterinary Medicine, Benha University
Abstract
The present study was planned to investigate the causative agent of Oreochromis niloticus summer mortality that resulted in significant economic losses in Port Said Governorate, Egypt during 2017.Many bacterial isolates were identified, among which Acinetobacter johnsonii has been selected to be the focus of the current study. Acinetobacter johnsonii_Egy was isolated and identified using the analytical profile index (API) 20 E, sequencing 16S ribossomal RNA gene fragment and phylogenetic analysis. A drug resistance evaluation revealed that A. johnsonii was resistant to ampicillin, gentamicin, lincomycin, nalidixic acid, tetracycline, and oxytetracycline among the tested antibiotics. Experimental infection was performed and challenged fish revealed similar clinical signs as seen in naturally infected ones. The disease appears to be highly infectious and lethal, causing 100% cumulative mortality during experimental infections. Virulence genes (fimH, traT, and iutA) and antibiotic resistance genes (qacED1, qnrS, sul1, dfrA, and aadA1) presence was confirmed in the A. johnsonii_Egy isolate.
In conclusion, A. johnsonii is highly infectious to O. niloticus and might have a great influence on transfer of the antibiotic resistance in aquaculture. According to the available knowledge, this study is first to report A. johnsonii as emerging opportunistic pathogen in the Egyptian Nile tilapia (in Port Said).
Keywords
Acinetobacter johnsonii_Egy; O. niloticus; Antibiotic susceptibility; Virulence
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