Effect of Extremely Low Frequency Magnetic Fieldin Growth, CM Case, Electric Conductivity and DNA of Aspergillus niger | ||||
| Egyptian Journal of Physics | ||||
| Article 6, Volume 49, Issue 1, 2021, Page 1-10 PDF (1.12 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejphysics.2020.29413.1042 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Sahar Aboneima   1; Mohammed M. El-Metwally2
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| 1physics department Faculty of Science Damanhour University | ||||
| 2Department of Botany and Microbiology, Faculty of Science, Damanhur University, Damanhur, Egypt | ||||
| Abstract | ||||
| Introduction: In the past years the extremely low frequency magnetic field (ELF-MF) of the millimeter range has been actively used in biology, medicine, biotechnology, in this study the impact of electromagnetic radiations in growth, carboxymethycellulase activity (CMCase), cell conductivity and RAPD DNA pattern of A. niger were investigated. Materials and Methods: the Aspergillus niger fungus treated with magnetic field at constant intensity 50Hz, 10mT at different exposure times for five days (2 h/day) Results: the total biomass dry weight inhibited by 72.2% after the first 2 h. ELF-MF showed also inhibition effect in CMCase activity of A. niger. The enzyme activity was decreased from 30 IU/ml to 22.5 IU/ml after only 2h of exposure and the treated fungus lost about 50% of its CMCase activity after 10 h.The treated fungus showed also decreased in conductivity of fungal cells from 1.78×10-1 to 0.51×10-5 Siemens/meter. The study extended to detect the changes in RAPD patterns of A. niger DNA before and after exposure to the 50 Hz frequency. In conclusion, ELF-MF can be used as a safe method in control the activity of highly resistant fungi as A. niger. | ||||
| Keywords | ||||
| Electromagnetic radiation; CMCase; electric conductivity; DNA; A. niger | ||||
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