ANTIBACTERIAL EFFECT OF FOUR IRRIGATING SOLUTIONS COMPARED TO THAT OF SODIUM HYPOCHLORITE (A COMPARATIVE IN-VITRO STUDY)
Arab, A., Negm, M., Youssef, S. (2016). ANTIBACTERIAL EFFECT OF FOUR IRRIGATING SOLUTIONS COMPARED TO THAT OF SODIUM HYPOCHLORITE (A COMPARATIVE IN-VITRO STUDY). EKB Journal Management System, 62(Issue 1 - January (Part 3)), 721-726. doi: 10.21608/edj.2016.95094
Ali Adel Arab; Maged Mohamed Negm; Salwa Ibrahim Youssef. "ANTIBACTERIAL EFFECT OF FOUR IRRIGATING SOLUTIONS COMPARED TO THAT OF SODIUM HYPOCHLORITE (A COMPARATIVE IN-VITRO STUDY)". EKB Journal Management System, 62, Issue 1 - January (Part 3), 2016, 721-726. doi: 10.21608/edj.2016.95094
Arab, A., Negm, M., Youssef, S. (2016). 'ANTIBACTERIAL EFFECT OF FOUR IRRIGATING SOLUTIONS COMPARED TO THAT OF SODIUM HYPOCHLORITE (A COMPARATIVE IN-VITRO STUDY)', EKB Journal Management System, 62(Issue 1 - January (Part 3)), pp. 721-726. doi: 10.21608/edj.2016.95094
Arab, A., Negm, M., Youssef, S. ANTIBACTERIAL EFFECT OF FOUR IRRIGATING SOLUTIONS COMPARED TO THAT OF SODIUM HYPOCHLORITE (A COMPARATIVE IN-VITRO STUDY). EKB Journal Management System, 2016; 62(Issue 1 - January (Part 3)): 721-726. doi: 10.21608/edj.2016.95094

ANTIBACTERIAL EFFECT OF FOUR IRRIGATING SOLUTIONS COMPARED TO THAT OF SODIUM HYPOCHLORITE (A COMPARATIVE IN-VITRO STUDY)

Egyptian Dental Journal
Article 16, Volume 62, Issue 1 - January (Part 3), January 2016, Page 721-726  XML PDF (243.76 K)
Document Type: Original Article
DOI: 10.21608/edj.2016.95094
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Authors
Ali Adel Arab* 1; Maged Mohamed Negm2; Salwa Ibrahim Youssef3
1Research Postgraduate Student
2Professor of Endodontics, Faculty of Oral and Dental Medicine, Cairo University
3Lecturer of microbiology, Faculty of Dentistry, October 6 University
Abstract
Aim : The aim of the present study was to evaluate the antimicrobial activity of four irrigating solutions against Enterococcus faecalis compared with sodium hypochlorite 5.25% namely: propolis extract, QMix, Chloroxylenol and CHX-Plus
Materials and methods: Enterococcus faecalis (batch no. 10541) thawed and incubated under aerobic condition. The harvested bacterial colonies are then spectrophotometrically calibrated to 5.9 x 104 (CFU/mL-1). Fifty specimens instrumented conventionally, reaching master apical file size #50. Twenty μL of the bacterial culture transferred to the canal lumen using sterile micropipette. All specimens were then incubated for 48 hours at 37 ˚C.
 
Results : Intracanal sample collection by paper points showed an insignificant difference between different irrigation solutions tested and positive control Group at p=0.001. Group 1 showed a median of 8.9*104(CFU/ml), where group 2, 3, 4 and positive control groups showed mean values of 2.2*104, 7.3*104, 8*104  and 3*104  (CFU/ml) respectively. Negative control group showed the highest significant values for colony forming units (118.4*104CFU/ml).
 
Conclusion : All groups except the saline produce of significant reduction of bacteria. Although QMix, NaOCl and chlorhexidine plus are capable of reducing bacterial count their effect is statistically insignificant
Keywords
Enterococcus faecalis; QMix; propolis extract; CHX-Plus; Chloroxylenol; NaOCl 5.25%
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