THE EFFECT OF OXIDATIVE STRESS ON MITOCHONDRIAL ULTRASTRUCTURE AND IMMUNOEXPRESSION OF ERK-1 AND HIF-1 ALPHA IN ORAL EPITHELIAL DYSPLASIA INDUCED IN EXPERIMENTAL ANIMALS | ||||
Egyptian Dental Journal | ||||
Article 22, Volume 62, Issue 1 - January (Part 3), January 2016, Page 765-775 PDF (2.62 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/edj.2016.95106 | ||||
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Authors | ||||
Nermine Raouf Amin1; Safa Fathy Abdel Ghany2; Radwa Hamed Hegazi3 | ||||
1Lecturer of Oral Pathology, Faculty of Oral and Dental Medicine, Cairo University | ||||
2Assistant Professor of Oral Pathology, Faculty of Oral and Dental Medicine, Cairo University | ||||
3Assistant Professor of Oral Biology, Faculty of Oral and Dental Medicine, Cairo University | ||||
Abstract | ||||
Background: Oxidative stress and accumulation of reactive oxygen species may play a role in the pathogenesis of cancer. They were reported in precancerous and cancerous cells. Methods: An animal model of carcinogenesis was used to detect the effect of oxidative stress on mitochondrial ultrastructure by transmission electron microscope and the levels of extracellular signal- regulated kinase-1(ERK-1) and hypoxia inducible factor-1 alpha (HIF-1α) by immunohistochemistry in mild and severe oral epithelial dysplasia (OED) induced in tongues of experimental mice. Results: We identified degeneration and enlargement of mitochondria in association with up- regulation of both ERK-1 and HIF-1α from normal control to mild OED and reached their highest values in severe OED. Conclusions: Degeneration and enlargement of mitochondria in association with up-regulation of both ERK-1 and HIF-1α can be indicative features of dysplastic changes and may act as early markers for malignancy. | ||||
Keywords | ||||
Oral epithelial dysplasia; oxidative stress; extracellular signal-regulated kinase -1 (ERK-1); hypoxia inducible factor-1 alpha (HIF-1α) | ||||
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