Isolation And Identification of Vibrio parahemolyticus Recovered From Some Shellfish Marketed In Zagazig City
Suelam, I., Raslan, A., Mohamed, M. (2013). Isolation And Identification of Vibrio parahemolyticus Recovered From Some Shellfish Marketed In Zagazig City. EKB Journal Management System, 41(2), 70-75. doi: 10.21608/zvjz.2013.95684
Iman IA Suelam; Amal RA Raslan; Mohamed EM Mohamed. "Isolation And Identification of Vibrio parahemolyticus Recovered From Some Shellfish Marketed In Zagazig City". EKB Journal Management System, 41, 2, 2013, 70-75. doi: 10.21608/zvjz.2013.95684
Suelam, I., Raslan, A., Mohamed, M. (2013). 'Isolation And Identification of Vibrio parahemolyticus Recovered From Some Shellfish Marketed In Zagazig City', EKB Journal Management System, 41(2), pp. 70-75. doi: 10.21608/zvjz.2013.95684
Suelam, I., Raslan, A., Mohamed, M. Isolation And Identification of Vibrio parahemolyticus Recovered From Some Shellfish Marketed In Zagazig City. EKB Journal Management System, 2013; 41(2): 70-75. doi: 10.21608/zvjz.2013.95684

Isolation And Identification of Vibrio parahemolyticus Recovered From Some Shellfish Marketed In Zagazig City

Zagazig Veterinary Journal
Article 7, Volume 41, Issue 2, June 2013, Page 70-75  XML PDF (2.61 MB)
Document Type: Original Article
DOI: 10.21608/zvjz.2013.95684
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Authors
Iman IA Suelam1; Amal RA Raslan1; Mohamed EM Mohamed2
1Academic Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University
2Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University
Abstract
The present investigation was undertaken to study the occurrence of V. parahaemolyticus in shellfish and to assess the genomic diversity within V. parahaemolyticus strains isolate using ERIC-PCR technique. A total of 130 samples of shellfish were collected from fish markets, Zagazig city included white shrimps (40); blue crabs (40) and mussels (50).V. parahaemolyticus were isolated and identified using standard bacteriological methods.ERIC-PCR was carried out for a total of 9 V. parahaemolyticus clones, representing white shrimp, blue crab and mussels (3 each).V. parahaemolyticus were isolated with the percentages of 17.5(7/40), 12.5(5/40) and 16.0(8/50), respectively. The overall percentage of the examined shellfish was 15.4(20/130).Overall, the ERIC-PCR results showed 6 separate clusters at similarity level 0.53. Isolates No 4, 2, 5, 3 grouped together (group I). Isolates No 1, 8, 9, 7 and 6 each clustered in their own groups (II, III, IV, V and VI, respectively). In addition, there is sub-clustering with group I at similarity level 0.66. The amplified bands were analyzed by dendrogram using Dice correlation coefficient. The phylogenetic analysis of tested strains exhibits high amount of heterogeneity.It could be concluded from the present study that V. parahaemolyticus are circulating among the marketed shellfish in Zagazig city. Variable V. parahaemolyticus clones indicate different sources of contamination and infections under the research conditions.
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