RELATIONSHIP BETWEEN FERTILIN  MRNA EXPRESSION AND THE FERTILIZING POTENTIALS OF HUMAN SPERMATOZOA
Zalata, A., Atwa, A., Mokhtar, N., khaled, M. (2016). RELATIONSHIP BETWEEN FERTILIN  MRNA EXPRESSION AND THE FERTILIZING POTENTIALS OF HUMAN SPERMATOZOA. EKB Journal Management System, 34(1-2), 51-66. doi: 10.21608/ejb.2016.9650
Adel Zalata; Amany Atwa; Naglaa Mokhtar; Mohamed khaled. "RELATIONSHIP BETWEEN FERTILIN  MRNA EXPRESSION AND THE FERTILIZING POTENTIALS OF HUMAN SPERMATOZOA". EKB Journal Management System, 34, 1-2, 2016, 51-66. doi: 10.21608/ejb.2016.9650
Zalata, A., Atwa, A., Mokhtar, N., khaled, M. (2016). 'RELATIONSHIP BETWEEN FERTILIN  MRNA EXPRESSION AND THE FERTILIZING POTENTIALS OF HUMAN SPERMATOZOA', EKB Journal Management System, 34(1-2), pp. 51-66. doi: 10.21608/ejb.2016.9650
Zalata, A., Atwa, A., Mokhtar, N., khaled, M. RELATIONSHIP BETWEEN FERTILIN  MRNA EXPRESSION AND THE FERTILIZING POTENTIALS OF HUMAN SPERMATOZOA. EKB Journal Management System, 2016; 34(1-2): 51-66. doi: 10.21608/ejb.2016.9650

RELATIONSHIP BETWEEN FERTILIN  MRNA EXPRESSION AND THE FERTILIZING POTENTIALS OF HUMAN SPERMATOZOA

The Egyptian Journal of Biochemistry and Molecular Biology
Article 5, Volume 34, 1-2, December 2016, Page 51-66  XML PDF (273.23 K)
Document Type: Original Article
DOI: 10.21608/ejb.2016.9650
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Authors
Adel Zalata1; Amany Atwa email 2; Naglaa Mokhtar2; Mohamed khaled3
1MEDICAL BIOCHEMISTRY DEPARTMENT FACULTY OF MEDICINE, MANSOURA UNIVERSITY, EGYPT
2MEDICAL BIOCHEMISTRY DEPARTMENT, FACULTY OF MEDICINE, MANSOURA UNIVERSITY, EGYPT
3DEPARTMENT OF DERMATOLOGY, VENEROLOGY & ANDROLOGY, FACULTY OF MEDICINE, MANSOURA UNIVERSITY, EGYPT
Abstract
Fertilin is a sperm surface protein that belongs to the ADAM (a
disintigrin and a metalloproteinase) family of integral membrane
proteins. It mediates adhesion and fusion between the sperm and egg
plasma membranes that may play important roles in mammalian
fertilization. This study aimed to explore the relation between
fertilization ability of human sperm and fertilin  mRNA expression in
non-fertile men. Expression of fertilin  gene mRNA was determined
by RT-PCR from 141 semen samples (infertile n=81 and normal
fertile n=60 men). Total RNA was extracted from sperm pellets.
cDNA was generated then amplified. Computer assisted semen
analysis and acrosine activity test were also performed. ELISA was
done to measure FSH, LH, testosterone and prolactin. The study
showed lowered sperm fertilin  expression in infertile group together
with decreased sperm motility and other sperm functions. Also, there
was significant positive correlation between fertilin  expression and
testosterone levels. Furthermore, normal morphology may influence
changes of fertilin  as shown by multiple regression analysis.
From this study it can be concluded that infertility which is associated
with decreased expression of sperm fertilin  could be due to failure of
sperm migration into the oviduct. Moreover, defective protease can
produce incomplete fertilin  processing with improper maturation of
sperm proteins. Here in this study showed possible direct role of
fertilin- in fertilization and also in sperm morphology and hence
sperm capicitation.
Keywords
Fertilin  expression; Infertility; hormonal profile; sperm function
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