Use of Molecular Markers to Identify Certain Stem Rust Resistance Genes in Thirteen Bread Wheat Cultivars Registered in Egypt | ||||
| Egyptian Journal of Phytopathology | ||||
| Article 13, Volume 42, Issue 1 - Serial Number 42, June 2014, Page 193-203 PDF (603.73 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejp.2014.96766 | ||||
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| Authors | ||||
| Mostafa El-Shamy* 1; Ayman Omar2 | ||||
| 1Wheat Dis. Res. Dept., Plant Pathol. Res. Inst., ARC, Giza, Egypt | ||||
| 2Botany Dept., Plant Pathol. & Biotechnol. Lab., Fac. Agric., Kafr El-Sheikh Univ. | ||||
| Abstract | ||||
| Wheat stem rust caused by Puccinia graminis f.sp. tritici (Pgt) is one of the most destructive diseases of wheat all over the world. In Egypt, in-spite of the absence of the new stem rust pathotype TTKS (syn. Ug99) and its variants TTKSK and TTKST, the disease takes place as a serious situation in the last few years with high disease severities on most of the common wheat cultivars during 2012-2014 growing seasons. A modified North American Pgt differential set representing the resistance genes Sr5, 21, 9e, 7b, 11, 6, 8a, 9g, 36, 9b, 30, 17, 9a, 9d, 10, Tmp, 24, 31, 38 and McN, respectively, as well as 10 supplemental Sr genes were evaluated in natural field infection. Adult resistance plants were detected on Srgenes Sr 5, Sr 9g, Sr 11, Sr 13, Sr 14, Sr 21, Sr 24, Sr 28 and Sr 38. Four specific markers were selected to detect the presence of the resistant stem rust monogenic lines including Sr13, Sr25, Sr26 and Sr31 in thirteen Egyptian bread wheat cultivars. These markers were XBE403950EST, PSY-DI, Sr26#43 STS and IAG95-STS, respectively. Stem rust resistance gene Sr26 was absent in all the tested cultivars, whereas Sr13, Sr25 and Sr31 were presented at various frequencies. The highest frequency was observed for Sr13 (100%), followed by Sr25 (84.61%) and Sr31 (46.15%). These results revealed that DNA marker for Sr26 is needed in the Egyptian cultivars and more effective genes must be identified and incorporate in adopted germplasms to face such disease. | ||||
| Keywords | ||||
| DNA markers; molecular marker; Puccinia graminis f.sp. tritici; Sr genes; Ug99 and wheat | ||||
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