INTEGRIN BETA 4 mRNA EXPRESSION LEVELS IN BRONCHIAL ASTHMA PATIENTS | ||||
| The Egyptian Journal of Biochemistry and Molecular Biology | ||||
| Article 3, Volume 35, 1-2, December 2017, Page 41-54 PDF (166.36 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejb.2017.9693 | ||||
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| Authors | ||||
Abeer ALrefai1; Rabab Elwahsh* 2; Nesreen Elhelbawy  3
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| 1Medical Biochemistry Faculty of Medicine, Menoufia University, Egypt | ||||
| 2Chest Department DEPARTMENTS FACULTY OF MEDICINE, MENOUFIA UNIVERSITY, EGYPT | ||||
| 3MEDICAL BIOCHEMISTRY AND INTERNAL MEDICINE DEPARTMENTS FACULTY OF MEDICINE, MENOUFIA UNIVERSITY, EGYP | ||||
| Abstract | ||||
| Integrin beta 4 (ITG4) is one of the integrin families that is engaged in the maintenance of the integrity of airway epithelial cells. The aim of this work was to evaluate the relationship between ITG4 mRNA expression level and asthma susceptibility; and to analyze the relevance of atopic asthma with the alteration of ITG4mRNA expression level.Seventy five asthmatic patients and thirty age and gender matched healthy controls were enrolled in this study. Serum total IgE was measured by ELISA and mRNA expression of ITG4 was assessed by reverse transcriptase PCR (RT-PCR) using real time PCR.. ITG4 mRNA expression was significantly down regulated with increased serum total IgE in patients with asthma compared to controls. Moreover, ITG4 expression was significantly reduced with increased total IgE in atopic asthmatics compared to non-atopic asthmatics.From this study, it could be concluded thatdown-regulation of ITG4 expression is associated with asthma susceptibility mainly in atopic cases irrespective of the degree of severity. | ||||
| Keywords | ||||
| Integrin 4; expression; Asthma; atopy and RT- PCR | ||||
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