A PRELIMINARY STUDY ON ENDOTHELIAL NITRIC OXIDE SYNTHASE INTRON 4a/b POLYMORPHISM IN ULCERATIVE COLITIS-ASSOCIATED COLORECTAL CANCER
GABALLAH, H., ISMAIL, S. (2015). A PRELIMINARY STUDY ON ENDOTHELIAL NITRIC OXIDE SYNTHASE INTRON 4a/b POLYMORPHISM IN ULCERATIVE COLITIS-ASSOCIATED COLORECTAL CANCER. EKB Journal Management System, 33(1-2), 16-33. doi: 10.21608/ejb.2015.9698
HANAA GABALLAH; SABER ISMAIL. "A PRELIMINARY STUDY ON ENDOTHELIAL NITRIC OXIDE SYNTHASE INTRON 4a/b POLYMORPHISM IN ULCERATIVE COLITIS-ASSOCIATED COLORECTAL CANCER". EKB Journal Management System, 33, 1-2, 2015, 16-33. doi: 10.21608/ejb.2015.9698
GABALLAH, H., ISMAIL, S. (2015). 'A PRELIMINARY STUDY ON ENDOTHELIAL NITRIC OXIDE SYNTHASE INTRON 4a/b POLYMORPHISM IN ULCERATIVE COLITIS-ASSOCIATED COLORECTAL CANCER', EKB Journal Management System, 33(1-2), pp. 16-33. doi: 10.21608/ejb.2015.9698
GABALLAH, H., ISMAIL, S. A PRELIMINARY STUDY ON ENDOTHELIAL NITRIC OXIDE SYNTHASE INTRON 4a/b POLYMORPHISM IN ULCERATIVE COLITIS-ASSOCIATED COLORECTAL CANCER. EKB Journal Management System, 2015; 33(1-2): 16-33. doi: 10.21608/ejb.2015.9698

A PRELIMINARY STUDY ON ENDOTHELIAL NITRIC OXIDE SYNTHASE INTRON 4a/b POLYMORPHISM IN ULCERATIVE COLITIS-ASSOCIATED COLORECTAL CANCER

The Egyptian Journal of Biochemistry and Molecular Biology
Article 2, Volume 33, 1-2, December 2015, Page 16-33  XML PDF (327.21 K)
Document Type: Original Article
DOI: 10.21608/ejb.2015.9698
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Authors
HANAA GABALLAH* 1; SABER ISMAIL2
1MEDICAL BIOCHEMISTRY DEPARTMENT FACULTY OF MEDICINE, TANTA UNIVERSITY, EGYPT
2TROPICAL MEDICINE DEPARTMENT, FACULTY OF MEDICINE, TANTA UNIVERSITY, EGYPT
Abstract
Cancer is one the most concerning complications of longstanding
ulcerative colitis (UC). This study was undertaken to investigate the
possible association between eNOS gene intron 4 polymorphism and
the development of UC-associated colorectal cancer. It was carried
out on 120 subjects; distributed as follows: 40 patients with UC
associated colorectal cancer, 40 patients with UC who did not develop
colorectal cancer and 40 control subjects. Genotypes (aa, bb, ab) for
eNOS gene intron 4 polymorphism were identified using amplified
fragment length polymorphism PCR. Plasma nitrate and nitrite levels
were used to estimate the amounts of endogenous nitric oxide
formation using nitric oxide colorimetric assay kit. Our preliminary
data revealed that, compared to the bb genotype, the aa and ab eNOS
genotypes were significantly associated with increased risk of
developing UC associated colorectal cancer. Meanwhile, in UC
associated - colorectal cancer and UC patients groups, plasma NO
levels were higher in patients with 4a4a/4a4b genotypes compared to
those with bb genotype. It can be concluded from our preliminary study that a allele variant of eNOS intron 4 polymorphism may be
associated with increased risk of development of UC associated
colorectal cancer, however, large scaled studies are needed to verify
these preliminary results.
 
Keywords
Endothelial Nitric Oxide Synthase; ulcerative colitis; colorectal cancer; intron 4 polymorphism
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