Fibroblast Growth Factor 7: Is it Efficient in Detecting Seminal Stains after Long Term Storage?
Gaballah, I., Badr, K., Rashed, L., Shalaby, E. (2020). Fibroblast Growth Factor 7: Is it Efficient in Detecting Seminal Stains after Long Term Storage?. EKB Journal Management System, 20(2), 85-94. doi: 10.21608/ejfsat.2020.27916.1139
Iman Fawzy Gaballah; Khaled Bayoumi Badr; Laila Ahmed Rashed; Ezz Abdelwahed Shalaby. "Fibroblast Growth Factor 7: Is it Efficient in Detecting Seminal Stains after Long Term Storage?". EKB Journal Management System, 20, 2, 2020, 85-94. doi: 10.21608/ejfsat.2020.27916.1139
Gaballah, I., Badr, K., Rashed, L., Shalaby, E. (2020). 'Fibroblast Growth Factor 7: Is it Efficient in Detecting Seminal Stains after Long Term Storage?', EKB Journal Management System, 20(2), pp. 85-94. doi: 10.21608/ejfsat.2020.27916.1139
Gaballah, I., Badr, K., Rashed, L., Shalaby, E. Fibroblast Growth Factor 7: Is it Efficient in Detecting Seminal Stains after Long Term Storage?. EKB Journal Management System, 2020; 20(2): 85-94. doi: 10.21608/ejfsat.2020.27916.1139

Fibroblast Growth Factor 7: Is it Efficient in Detecting Seminal Stains after Long Term Storage?

The Egyptian Journal of Forensic Sciences and Applied Toxicology
Article 8, Volume 20, Issue 2, June 2020, Page 85-94  XML PDF (473.36 K)
Document Type: Original Article
DOI: 10.21608/ejfsat.2020.27916.1139
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Authors
Iman Fawzy Gaballah email orcid 1; Khaled Bayoumi Badr2; Laila Ahmed Rashed3; Ezz Abdelwahed Shalaby4
1Department of Forensic Medicine and Clinical Toxicology .. Kasr Alainy School of Medicine .. Cairo University
2Department of Forensic Medicine and Clinical Toxicology - Kasr Alainy school of Medicine - Cairo university
3Department of Biochemisty - Kasr Alainy School of Medicine - Cairo University
4Department of Forensic Medicine and Clinical Toxicology - Kasr Alainy School of Medicine - Cairo University
Abstract
ABSTRACT
INTRODUCTION: proper detection and analysis of the biological evidence is a crucial matter for crime scene reconstruction and the most promising method for tissue determination is the analysis of differential DNA methylation patterns according to cell or tissue type. These patterns are chemically and biologically more stable thus representing a more reliable identification tool. Identification of semen and sperms are particularly important, especially in sexual assaults, where sperms are the source of DNA in the human ejaculate. In practice, sperms isolated from sexual assault evidence lose most of their distinctive sub-cellular organelles upon which morphological identification depends. AIM OF THE RESEARCH: to identify stability of the FGF7 marker in stored seminal specimens. MATERIAL and METHODS: 15 seminal specimens stored for over 10 years were used along with 5 fresh samples for FGF7 assessment. RESULTS and CONCLUSION: DNA methylation proved to be a stable marker for both fresh semen and stored semen samples.
Keywords
KEYWORDS epigenetics; DNA methylation; differential DNA methylation; FGF7; forensic detection of sperms; bisulphite conversion
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