EVALUATION OF SOME RICE VARIETIES FOR THEIR ABILITIES TO IMMATURE EMBRYO CULTURE UNDER SALINITY STRESS | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 1, Volume 34, Issue 9, September 2009, Page 9185-9192 PDF (587.23 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2009.99285 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| M.M. Abd El-Maksoud1; A.E. Draz2; Sara A. El-Leithy2 | ||||
| 1Genetic Dept. Faculty of Agric., Mansoura Univ., Mansoura, Egypt. | ||||
| 2Rice Res. and Training Center (RRTC), Sakha, Kafr El-Sheikh, Egypt. | ||||
| Abstract | ||||
| This study was carried out to evaluate some rice varieties for their ability to immature embryo culture under salt stress condition. Therefore, three varieties were used as the donor plants for immature embryo culture in order to assessment their abilities for response under salinity stress. The recorded data on the embryo response percentage, shoot bud percentage and plantlet percentage were subjected for statistical analyses. The test of significance of the mean squares of genotypes at the three levels of NaCl except for embryo response percentage at control level, indicated the presence of significant differences among these genotypes for all in vitro traits. This finding indicated that these genotypes were varied in their response to immature embryo culture. Furthermore, levels and genotype × levels interaction mean squares were highly significant in plantlet percentage trait. This result indicated that these genotypes gave different response for different NaCl levels. At level 2000 ppm, although the greatest mean values for shoot bud percentage was recorded in Giza 177 with the mean of 69.98% which was not significantly differed than Giza 182 (64.00%). Also, the greatest mean values for plantlet percentage was recorded in Giza 182 with the mean of 58.79%. On the other hand, the lowest shoot bud percentage was observed in Giza 178 with the mean of 44.80% as well as, Giza 177 which recorded the lowest values for plantlet percentage with the mean of 34.58%. Regarding the third level of NaCl 4000 ppm, the best variety with the highest mean values for shoot bud and plantlet percentages was Giza182. This finding indicated that Giza 182 was highly tolerant to NaCl concentration at in vitro levels and may produce somaclones with NaCl tolerance genes | ||||
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