Paraoxonase1 Activity and Paraoxonase1 Q192R Gene Polymorphism in Systemic Lupus Erythematosus Patients as a Risk Factor for Atherosclerosis
Nabih, M., El-Shehaby, A., Heshmat, H. (2014). Paraoxonase1 Activity and Paraoxonase1 Q192R Gene Polymorphism in Systemic Lupus Erythematosus Patients as a Risk Factor for Atherosclerosis. EKB Journal Management System, 2(1), 27-35. doi: 10.21608/ejrci.2014.9984
Mona I. Nabih; Amal R. El-Shehaby; Hussein Heshmat. "Paraoxonase1 Activity and Paraoxonase1 Q192R Gene Polymorphism in Systemic Lupus Erythematosus Patients as a Risk Factor for Atherosclerosis". EKB Journal Management System, 2, 1, 2014, 27-35. doi: 10.21608/ejrci.2014.9984
Nabih, M., El-Shehaby, A., Heshmat, H. (2014). 'Paraoxonase1 Activity and Paraoxonase1 Q192R Gene Polymorphism in Systemic Lupus Erythematosus Patients as a Risk Factor for Atherosclerosis', EKB Journal Management System, 2(1), pp. 27-35. doi: 10.21608/ejrci.2014.9984
Nabih, M., El-Shehaby, A., Heshmat, H. Paraoxonase1 Activity and Paraoxonase1 Q192R Gene Polymorphism in Systemic Lupus Erythematosus Patients as a Risk Factor for Atherosclerosis. EKB Journal Management System, 2014; 2(1): 27-35. doi: 10.21608/ejrci.2014.9984

Paraoxonase1 Activity and Paraoxonase1 Q192R Gene Polymorphism in Systemic Lupus Erythematosus Patients as a Risk Factor for Atherosclerosis

Egyptian Journal of Rheumatology and Clinical Immunology
Article 7, Volume 2, Issue 1, January 2014, Page 27-35  XML PDF (175.63 K)
Document Type: Original Article
DOI: 10.21608/ejrci.2014.9984
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Authors
Mona I. Nabih1; Amal R. El-Shehaby2; Hussein Heshmat3
1Departments of Internal Medicine, Cairo University; Egypt.
2Departments of Medical Biochemistry, Cairo University; Egypt
3Departments of Cardiology, Cairo University; Egypt
Abstract
Background: Accelerated atherosclerosis has become an important cause of death in Systemic Lupus Erythematosus (SLE) patients. The protective role of HDL-C against atherosclerosis is believed to be mainly due to the enzyme paraoxonase1 (PON1) which functions to prevent LDL-C from peroxidation. Decreased PON1 activity has been observed in SLE patients.
Objectives: In the present study, we aimed to determine the PON1 activity and PON1 Q192R polymorphism in SLE patients and to study their relation to premature atherosclerosis. Methods: 40 SLE patients and 20 age & sex matched controls were included. PON1 activity was determined by paraoxon substrate. PON1 genotyping was conducted by PCR followed by polymorphism-specific restriction enzyme digestion and gel electrophoresis. Atherosclerosis was assessed by intima-media thickness (IMT). Results: Significantly lower PON1 activity and higher IMT was found in SLE patients compared to controls (p<0.05). Significant negative correlation was observed between PON1 activity and IMT, SLEDAI score, total cholesterol and LDL-C. There was no significant difference in the prevalence of any of the PON1 Q192R polymorphisms in SLE patients compared to controls. PON1 activity was lowest in the QQ, intermediate in QR and highest in RR group (p<0.05) and the mean IMT was highest in RR, intermediate in QR and lowest in QQ group (p<0.05). Conclusion: Although there was no association between any of the PON1Q192R polymorphism and SLE, PON1 activity was significantly decreased in SLE patients and was significantly correlated with the severity of atherosclerotic lesions. And thus, it might be involved in the pathogenesis of atherosclerosis in SLE patients.
Keywords
SLE; Paraoxonase Activity; PON1Q192R polymorphism; Atherosclerosis
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