The efficiency of Intra-articular injection of amphotericin B in inducing arthritis in experimental Equine model
A, S., A, M., O, E., A, M. (2011). The efficiency of Intra-articular injection of amphotericin B in inducing arthritis in experimental Equine model. EKB Journal Management System, 59(3), 397-415. doi: 10.21608/vmjg.2011.368303
Shaman A; Mokbel A; El-Tookhy O; Mostafa A. "The efficiency of Intra-articular injection of amphotericin B in inducing arthritis in experimental Equine model". EKB Journal Management System, 59, 3, 2011, 397-415. doi: 10.21608/vmjg.2011.368303
A, S., A, M., O, E., A, M. (2011). 'The efficiency of Intra-articular injection of amphotericin B in inducing arthritis in experimental Equine model', EKB Journal Management System, 59(3), pp. 397-415. doi: 10.21608/vmjg.2011.368303
A, S., A, M., O, E., A, M. The efficiency of Intra-articular injection of amphotericin B in inducing arthritis in experimental Equine model. EKB Journal Management System, 2011; 59(3): 397-415. doi: 10.21608/vmjg.2011.368303

The efficiency of Intra-articular injection of amphotericin B in inducing arthritis in experimental Equine model

Veterinary Medical Journal (Giza)
Volume 59, Issue 3, July 2011, Page 397-415  XML PDF (3.23 MB)
Document Type: Original Article
DOI: 10.21608/vmjg.2011.368303
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Authors
Shaman A* 1; Mokbel A2; El-Tookhy O3; Mostafa A4
1Surgery, Anesthesiology and Radiology Dept., Faculty of Veterinary Medicine. Cairo Univ., Egypt.
2Rheumatology and Rehabilitation, Faculty of Medicine. Cairo Univ., Egypt.
3Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo Univ., Egypt
4Pathology, National Cancer Institute. Cairo Univ., Egypt.
Abstract
 This study was carried out to verify the clinical efficiency of amphotericin B to induce arthritic changes when injected intra-articularly in equines and to correlate to the histopathological findings. Twenty mg Amphotericin B were injected in right radio- carpal joints of 9 donkeys. Animals were grouped equally into three groups based on the observation time period after injection-(3, 6 and 9 weeks) to evaluate the different degrees of arthritis, Clinical, radiological, histopathological assessments together with synovial fluid analysis were done.
       The arthritic changes were observed in all groups. Acute inflammatory reaction developed few hours post injection manifested by lameness, swelling of the joint with significant increase in total protein, enzymes, cell count and mucin precipitation. Macroscopic findings were limited to discoloration of the articular cartilage, few hemorrhagic areas in the joint capsule with slight roughness of the articular surface in old cases. Radiographic arthritic evidences included synovial capsuler swelling in acute cases and narrowing of the joint space in chronic ones. Histopathological changes revealed degeneration and thinning of both cartilage and bone, synovitis, capsulitis and osteophytes formation. The cartilage matrix lost its architecture with fibrous replacement.
In conclusion: different degrees of persistent arthritis could be achieved with a single IA injection of amphotericin B in Equine. Joints of these experimental animals are considered as a model of arthritis and can be used for testing different recent treatment techniques.
Keywords
amphotericin B; arthritis; equine; model; synovial analysis
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