The Creation and Validation of a New High-Performance Liquid Chromatography Method for Analyzing Pyridostigmine Bromide and its Degradation Products in a Pharmaceutical Formulation | ||
| Sinai International Scientific Journal | ||
| Volume 1, Issue 2, October 2024, Pages 35-48 PDF (516.62 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/sisj.2024.389246 | ||
| Authors | ||
| Sherif M. Fawzy* 1; Mohamed A. Helal2; Lobna M. Abdel-Aziz3; Ismail Salama4 | ||
| 1Pharmaceutical Chemistry Department, Faculty of Pharmacy, Sinai University, Kantara, Egypt | ||
| 2Biomedical Sciences Program, Zewail City of Science and Technology, Giza, Egypt | ||
| 3Medicinal Chemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt | ||
| 4Medicinal Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt | ||
| Abstract | ||
| A novel, rapid, sensitive, and validated high-performance liquid chromatographic (HPLC) technique, with high selectivity for stability indication, has been employed to determine pyridostigmine bromide and its degradation products (3-hydroxy-1- methylpyidin-1-ium bromide and 3,4-dioxo-3,4-dihydropyeidin-1-ium) in a pharmaceutical formulation. Pyridostigmine bromide and its degradation products were identified using combinations of acetonitrile and 0.01M acetate buffer (pH=6) in a ratio of 5:95 (v/v), through flow rates of about 1 ml/min at room temperature, employing a diode array detector. The linear range was determined to be 27-135 µg/ml. Our findings indicate that pyridostigmine bromide is unstable in alkaline conditions but more stable in acidic environments and susceptible to oxidation. | ||
| Keywords | ||
| HPLC; DAD; UPLC; PYR | ||
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