Leachate emanating from a municipal solid waste dumpsite is a hotspot of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli harboring CTX-M β-lactamase | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 22 November 2024 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2024.303867.2073 | ||||
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| Authors | ||||
Abimbola O Adekanmbi   1, 2; Akeem G. Rabiu 3; Olabisi C. Akinlabi4; Daniel J. Ajose 5, 6; Adedolapo V. Olaposi1, 7; Ayomikun E. Aderounmu 1; Omowunmi A. Banjo8; Temitope A. Ajamu1, 7; Busayo F. Tofio1, 7; Hassan A. Sanuth9
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| 1Environmental Microbiology and Biotechnology Laboratory, Department of Microbiology, University of Ibadan, Ibadan, Oyo state, Nigeria | ||||
| 2Molecular Biology and Biotechnology Laboratory, Department of Microbiology, University of Ibadan, Ibadan, Oyo State, Nigeria. Swammerdam Institute of Life Sciences (SILS), University of Amsterdam, Netherlands | ||||
| 3Department of Microbiology, Federal University of Health Sciences, Ila-Orangun, Osun State, Nigeria | ||||
| 4Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Oyo state, Nigeria | ||||
| 5Antimicrobial Resistance and Phage Biocontrol Research Group (AREPHABREG), Department of Microbiology, School of Biological Sciences, Faculty of Natural and Agricultural Sciences, North-West University, Mmabatho 2735, Private Mail Bag X2046, Mmabatho, South Africa | ||||
| 6Food Security and Safety Focus Area, Faculty and Natural Agricultural Sciences, North-West University, Mmabatho 2735, Private Mail Bag X2046, Mmabatho, South Africa | ||||
| 7Molecular Biology and Biotechnology Laboratory, Department of Microbiology, University of Ibadan, Ibadan, Oyo State, Nigeria | ||||
| 8Department of Microbiology, Olabisi Onabanjo University, Ago-Iwoye, Nigeria | ||||
| 9Sanitation Services Department, Lagos State Ministry of the Environment and Water Resources, Lagos state, Nigeria. | ||||
| Abstract | ||||
| Background: Antibiotic resistance constitutes a key challenge to global public health and is on an alarming rise. In Nigeria, this quagmire has been extensively studied in clinical settings but not in the environmental sector, particularly in landfill leachates. Aim: We aimed to assess the susceptibility profile of Escherichia coli from leachate obtained from Lapite dumpsite, to determine the potential of dumpsite leachate as a source of ESBL-producing Escherichia coli. Methods: Cefotaxime-resistant Escherichia coli was isolated on cefotaxime-enriched chromogenic medium and identified via PCR using uidA gene. The confirmed isolates from the pool were assessed for susceptibility to antibiotics using the disc-diffusion method, while the phenotypic detection of ESBL production was done using the double disc synergy test. Detection of ESBL genes was done using primer-specific PCR. Results: Twenty-three ESBL-producing E. coli were obtained, and they showed 100% resistance to cefotaxime and cefpodoxime, and 60.87% resisting imipenem (a carbapenem). However, none of the isolates was resistant to amoxicillin-clavulanate. The resistance to the other antibiotics tested was: gentamicin (4.35%), ciprofloxacin (17.39%), nalidixic acid (26.09%), ceftazidime (26.09%) and chloramphenicol (30.43%). Seventy-four percent of the isolates had multiple antibiotic resistance index (MARI) greater than 0.3, with 30.4% (n=7/23) exhibiting multidrug resistance phenotypes. All the isolates carried blaCTX-M, but none harboured blaSHV or blaTEM. Conclusion: Landfill leachates could contribute to the evolution of antibiotic-resistant ESBL-producing E. coli harboring ESBL genes. There should effective precautionary methods in place to prevent dumpsite leachates from flowing into adjoining environment to safeguard public and environmental health. | ||||
| Keywords | ||||
| Lapite dumpsite; ESBL-producing Escherichia coli; ESBL genes; antibiotic resistance; multidrug resistance | ||||
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