Potential Application of Saccharomyces cerevisiae as an Antioxidant Agent for Broiler Chickens – A Brief Overview

ABD El-HAMID, I.S., 2024. Influence of Spirulina platensis supplementation alone or mixed with live yeast on blood constituents and oxidative status of Damascus goats and their new born. Journal of Applied Veterinary. Sciences, 9 (1), 1-12.https://doi.org/10.21608/javs.2023.231998.1266

ABO-SRIEA, T. M., ISMAEL, E., SOBHI, B. M., HASSAN, N. H., ELLEITHY, E. M., OMAR, S. A., and RAMADAN, A., 2024. Impact of dietary-nucleotides and Saccharomyces cerevisiae-derivatives on growth-performance, antioxidant-capacity, immune-response, small-intestine histomorphometry, caecal-Clostridia, and litter-hygiene of broiler-chickens treated with florfenicol. International Journal of Veterinary Science and Medicine, 12(1), 11-24. https://doi.org/10.1080/23144599.2024.2324411

AFRILIANA, A., PURNOMO, B. L., and WITONO, Y., 2023. Antioxidant activity of fermented inferior Jember Robusta coffee beans using Saccharomyces cerevisiae as a starter in the semi-carbonic maceration technique. Asian Journal of Food Research and Nutrition, 2(4), 451-461. https://journalajfrn.com/index.php/AJFRN/article/view/69

AHIWE, E. U., DOS SANTOS, T. T., GRAHAM, H., and IJI, P. A., 2021. Can probiotic or prebiotic yeast (Saccharomyces cerevisiae) serve as alternatives to in-feed antibiotics for healthy or disease-challenged broiler chickens?. a review. Journal of Applied Poultry Research, 30(3), 100164. https://doi.org/10.1016/j.japr.2021.100164

AL-ABDULLATIF, A. A., ALHOTAN, R. A., AL-BADWI, M. A., DONG, X., KETTUNEN, H., VUORENMAA, J., and AZZAM, M. M., 2024. Effects of hydrolyzed yeast on growth performance, intestinal redox homeostasis, and woody breast myopathy in heat-stressed broilers. Frontiers in Veterinary Science, 11, 1484150. https://doi.org/10.3389/fvets.2024.1484150

ALAGBE, E. O., SCHULZE, H., and ADEOLA, O., 2023. Growth performance, nutrient digestibility, intestinal morphology, cecal mucosal cytokines and serum antioxidant responses of broiler chickens to dietary enzymatically treated yeast and coccidia challenge. Journal of Animal Science and Biotechnology, 14(1), 57. https://doi.org/10.1186/s40104-023-00846-z

AL-BAADANI, H. H., ALHARTHI, A. S., ABBAS, N. I., QASEM, A. A., SALEH, A., and IBRAHEEM, M. A., 2025. Effect of activated and inactivated Saccharomyces cerevisiae as alternative to antibiotic growth promoter on the performance and health of broilers infected with Clostridium perfringens. Italian Journal of Animal Science, 24(1), 43-52. https://doi.org/10.1080/1828051X.2024.2441349

ALUWONG, T., KAWU, M., RAJI, M., DZENDA, T., GOVWANG, F., SINKALU, V., and AYO, J., 2013. Effect of yeast probiotic on growth, antioxidant enzyme activities and malondialdehyde concentration of broiler chickens. Antioxidants, 2(4), 326-339. https://doi.org/10.3390/antiox2040326

ANGGRAENI, D., SUNARTI, D., and SUGIHARTO, S., 2025. Effects of Saccharomyces cerevisiae-fermented infertile egg flour on growth performance, antioxidant status and carcass of broilers raised in high density pens. Pakistan Journal of Agricultural Science, 62(1), 131-139. https://doi.org/10.21162/PAKJAS/25.375

APALOWO, O. O., EKUNSEITAN, D. A., and FASINA, Y. O., 2024. Impact of heat stress on broiler chicken production. Poultry, 3(2), 107-128. https://doi.org/10.3390/poultry3020010

ARISTIDES, L. G. A., VENANCIO, E. J., ALFIERI, A. A., OTONEL, R. A. A., FRANK, W. J., and OBA, A. 2018. Carcass characteristics and meat quality of broilers fed with different levels of Saccharomyces cerevisiae fermentation product. Poultry science, 97(9), 3337-3342. https://doi.org/10.3382/ps/pey174

ATMACA, G., 2004. Antioxidant effects of sulfur-containing amino acids. Yonsei Medical Journal, 45(5), 776-788. https://doi.org/10.3349/ymj.2004.45.5.776

ATTIA, Y. A., AL-KHALAIFAH, H., EL-HAMID, A., HATEM, S., AL-HARTHI, M. A., ALYILEILI, S. R., and EL-SHAFEY, A. A., 2022. Antioxidant status, blood constituents and immune response of broiler chickens fed two types of diets with or without different concentrations of active yeast. Animals, 12(4), 453. https://doi.org/10.3390/ani12040453

AYLWARD, B. A., JOHNSON, C. N., PERRY, F., WHELAN, R., and ARSENAULT, R. J., 2024. Modern broiler chickens exhibit a differential gastrointestinal immune and metabolic response to repeated CpG injection relative to a 1950s heritage broiler breed. Frontiers in Physiology, 15, 1473202. https://doi.org/10.3389/fphys.2024.1473202

BAI, K., HUANG, Q., ZHANG, J., HE, J., ZHANG, L., and WANG, T., 2017. Supplemental effects of probiotic Bacillus subtilis fmbJ on growth performance, antioxidant capacity, and meat quality of broiler chickens. Poultry Science, 96(1), 74-82. https://doi.org/10.3382/ps/pew246

BILAL, R. M., HASSAN, F. U., SAEED, M., RAFEEQ, M., ZAHRA, N., FRAZ, A., SAEED, S., KHAN, M. A., MAHGOUB, H. A. M., FARAG, M. R., and ALAGAWANY, M., 2021. Role of yeast and yeast-derived products as feed additives in broiler nutrition. Animal Biotechnology, 34(2), 392-401. https://doi.org/10.1080/10495398.2021.1942028

CHANDRA, M. A., SYAMSU, K., AMBARSARI, L., FATIMAH, N., and NURCHOLIS, W., 2024. Increasing polyphenol antioxidant in Orthosiphon stamineus Benth leaves with fermentation extraction by Saccharomyces cerevisiae ATCC-9763. Journal of Applied Biology and Biotechnology, 12(1), 111-116. https://doi.org/10.7324/JABB.2024.149026

CHEN, F., ZHU, L., QIU, H., and QIN, S., 2017. Selenium‐enriched Saccharomyces cerevisiae improves growth, antioxidant status and selenoprotein gene expression in Arbor Acres broilers. Journal of Animal Physiology and Animal Nutrition, 101(2), 259-266. https://doi.org/10.1111/jpn.12571

CHUANG, W. Y., LIN, L. J., HSIEH, Y. C., CHANG, S. C., and LEE, T. T., 2021. Effects of Saccharomyces cerevisiae and phytase co-fermentation of wheat bran on growth, antioxidation, immunity and intestinal morphology in broilers. Animal Bioscience, 34(7), 1157. https://doi.org/10.5713/ajas.20.0399

CHUANG, W. Y., LIN, W. C., HSIEH, Y. C., HUANG, C. M., CHANG, S. C., and LEE, T. T., 2019. Evaluation of the combined use of Saccharomyces cerevisiae and Aspergillus oryzae with phytase fermentation products on growth, inflammatory, and intestinal morphology in broilers. Animals, 9(12), 1051. https://doi.org/10.3390/ani9121051

COLOVIC, M. B., VASIC, V. M., DJURIC, D. M., and KRSTIC, D. Z., 2018. Sulphur-containing amino acids: protective role against free radicals and heavy metals. Current Medicinal Chemistry, 25(3), 324-335. https://doi.org/10.2174/0929867324666170609075434

DARWESH, O. M., EWEYS, A. S., ZHAO, Y. S., and MATTER, I. A., 2023. Application of environmental-safe fermentation with Saccharomyces cerevisiae for increasing the cinnamon biological activities. Bioresources and Bioprocessing, 10(1), 12. https://doi.org/10.1186/s40643-023-00632-9

EJUAMA, C. K., ONUSIRIUKA, B. C., BAKARE, V., NDIBE, T. O., YAKUBU, M., and ADEMU, E. G., 2021. Effect of Saccharomyces cerevisiae-induced fermentation on the antioxidant property of Roselle Calyx aqueous extract. European Journal of Biology and Biotechnology, 2(3), 33-38. https://doi.org/10.24018/ejbio.2021.2.3.201

FAKRUDDIN, M. D., HOSSAIN, M. N., AND AHMED, M. M., 2017. Antimicrobial and antioxidant activities of Saccharomyces cerevisiae IFST062013, a potential probiotic. BMC Complementary and Alternative Medicine, 17, 1-11. https://doi.org/10.1186/s12906-017-1591-9

GHOSAL, S., BHATTACHARYYA, D. K., and BHOWAL, J., 2021. Effect of Saccharomyces cerevisiae fermentation process on the phenolic content, flavonoid content and antioxidant properties of flaxseeds. In Advances in Bioprocess Engineering and Technology: Select Proceedings ICABET 2020 (pp. 119-131). Springer Singapore. https://doi.org/10.1007/978-981-15-7409-2_12

GRŽINIĆ, G., PIOTROWICZ-CIEŚLAK, A., KLIMKOWICZ-PAWLAS, A., GÓRNY, R. L., ŁAWNICZEK-WAŁCZYK, A., PIECHOWICZ, L., and WOLSKA, L., 2023. Intensive poultry farming: A review of the impact on the environment and human health. Science of the Total Environment, 858, 160014. https://doi.org/10.1016/j.scitotenv.2022.160014

GUO, Y., ZHANG, J., LI, X., WU, J., HAN, J., YANG, G., and ZHANG, L., 2023. Oxidative stress mediated immunosuppression caused by ammonia gas via antioxidant/oxidant imbalance in broilers. British Poultry Science, 64(1), 36-46. https://doi.org/10.1080/00071668.2022.2122025

HASHEMITABAR, S. H., and HOSSEINIAN, S. A., 2024. The comparative effects of probiotics on growth, antioxidant indices and intestinal histomorphology of broilers under heat stress condition. Scientific Reports, 14(1), 23471. https://doi.org/10.1038/s41598-024-66301-9

HASSAN, H. M., 2011. Antioxidant and immunostimulating activities of yeast (Saccharomyces cerevisiae) autolysates. World Applied Science Journal, 15(8), 1110-9. https://www.idosi.org/wasj/wasj15(8)11/11.pdf

HE, T., MA, J., MAHFUZ, S., ZHENG, Y., LONG, S., WANG, J., and PIAO, X., 2022. Dietary live yeast supplementation alleviates transport‐stress‐impaired meat quality of broilers through maintaining muscle energy metabolism and antioxidant status. Journal of the Science of Food and Agriculture, 102(10), 4086-4096. https://doi.org/10.1002/jsfa.11758

HE, T., MAHFUZ, S., PIAO, X., WU, D., WANG, W., YAN, H., and LIU, Y., 2021. Effects of live yeast (Saccharomyces cerevisiae) as a substitute to antibiotic on growth performance, immune function, serum biochemical parameters and intestinal morphology of broilers. Journal of Applied Animal Research, 49(1), 15-22. https://doi.org/10.1080/09712119.2021.1876705

HEINSOHN, Z., BROWN, A., SOBOTIK, E., HOUSE, G., STIEWERT, A., CHANEY, W. E., and ARCHER, G. S., 2024. Evaluating the effects of feeding a concentrated Saccharomyces cerevisiae fermentation product on the performance and stress susceptibility of broiler chickens. Poultry, 3(1), 57-65. https://doi.org/10.3390/poultry3010006

HOU, L., QIU, H., SUN, P., ZHU, L., CHEN, F., and QIN, S., 2020. Selenium-enriched Saccharomyces cerevisiae improves the meat quality of broiler chickens via activation of the glutathione and thioredoxin systems. Poultry Science, 99(11), 6045-6054. https://doi.org/10.1016/j.psj.2020.07.043

JELENA, J., and YUSTIANTARA, P. S., 2021. Antioxidant activity of fermented coffee beans. Pharmacy Reports, 1(2), 25-25. https://doi.org/10.51511/pr.25

LIANG, Z., HUANG, Y., ZHANG, P., and FANG, Z., 2023. Impact of fermentation on the structure and antioxidant activity of selective phenolic compounds. Food Bioscience, 56, 103147. https://doi.org/10.1016/j.fbio.2023.103147

LIN, J., COMI, M., VERA, P., ALESSANDRO, A., QIU, K., WANG, J., and ZHANG, H. J., 2023. Effects of Saccharomyces cerevisiae hydrolysate on growth performance, immunity function, and intestinal health in broilers. Poultry Science, 102(1), 102237. https://doi.org/10.1016/j.psj.2022.102237

LUO, G. M., REN, X. J., LIU, J. Q., MU, Y., and SHEN, J. C., 2003. Towards more efficient glutathione peroxidase mimics: Substrate recognition and catalytic group assembly. Current Medicinal Chemistry, 10(13), 1151-83. https://doi.org/10.2174/0929867033457502

MAKKY, E. A., ALMATAR, M., MAHMOOD, M. H., TING, O. W., and QI, W. Z., 2021. Evaluation of the antioxidant and antimicrobial activities of ethyl acetate extract of Saccharomyces cerevisiae. Food technology and biotechnology, 59(2), 127-136. https://doi.org/10.17113/ftb.59.02.21.6658

MOHAMED, H. E., IBRAHIM, W. A., and GAAFAR, R. E., 2022. Impact of Moringa olifera leaves or Saccharomyces supplementation on carcass quality, mRNA of heat shock proteins and antioxidants in broilers exposed to heat stress. SVU-International Journal of Veterinary Sciences, 5(4), 193-227. https://doi.org/10.21608/svu.2022.167661.1232

NAJAFI, P., RAMIAH, S. K., AMAT JAJULI, N., FARJAM, A. S., ZULKIFLI, I., O’REILY, E., ECKERSALL, D., and AMIR, A. A., 2015. Environmental temperature and stocking density effects on acute phase proteins, heat shock protein 70, circulating corticosterone and performance in broiler chickens. International Journal of Biometeorology, 59(11), 1577-1583. https://doi.org/10.1007/s00484-015-0964-3

NELSON, J. R., SOBOTIK, E. B., ATHREY, G., and ARCHER, G. S., 2020. Effects of supplementing yeast fermentate in the feed or drinking water on stress susceptibility, plasma chemistry, cytokine levels, antioxidant status, and stress- and immune-related gene expression of broiler chickens. Poultry Science, 99, 3312-3318. https://doi.org/10.1016/j.psj.2020.03.037

OBIANWUNA, U. E., CHANG, X., OLEFORUH-OKOLEH, V. U., ONU, P. N., ZHANG, H., QIU, K., and WU, S., 2024. Phytobiotics in poultry: revolutionizing broiler chicken nutrition with plant-derived gut health enhancers. Journal of Animal Science and Biotechnology, 15(1), 169. https://doi.org/10.1186/s40104-024-01101-9

PRABHU, A., GARG, Y., CHITYALA, S., and VENKATA, D., V., 2016. Improvement of phytonutrients and antioxidant properties of wheat bran by yeast fermentation. Current Nutrition and Food Science, 12(4), 249-255. https://doi.org/10.2174/1573401312666160830155812

QIU, S., YAO, K., SUN, J., LIU, S., and SONG, X., 2025. Impact of fermentation by Saccharomyces cerevisiae on the macronutrient and in vitro digestion characteristics of Chinese noodles. Food Chemistry, 462, 140967. https://doi.org/10.1016/j.foodchem.2024.140967

RAKNGAM, S., ZHU, Y., OKRATHOK, S., PUKKUNG, C., and KHEMPAKA, S., 2024. Enhancing heat stress resilience in broiler chickens through the use of probiotics and postbiotics: a review. Tropical Animal Science Journal, 47(4), 538-548. https://doi.org/10.5398/tasj.2024.47.4.538

RAMOS-GONZÁLEZ, E. J., BITZER-QUINTERO, O. K., ORTIZ, G., HERNÁNDEZ-CRUZ, J. J., and RAMÍREZ-JIRANO, L. J., 2024. Relationship between inflammation and oxidative stress and its effect on multiple sclerosis. Neurologia, 39(3), 292-301. https://doi.org/10.1016/j.nrleng.2021.10.010

RIBER, A. B., and WURTZ, K. E., 2024. Impact of growth rate on the welfare of broilers. Animals, 14(22), 3330. https://doi.org/10.3390/ani14223330

RYBARCZYK, A., HARAF, G., TOKARCZYK, G., TELESZKO, M., TOBOLSKA, I., and BIENKIEWICZ, G., 2024. Effect of distiller’s yeast in feed on texture, fatty acid profile and antioxidant properties of breast muscle of broiler chickens. Agricultural and Food Science, 33, 2. https://doi.org/10.23986/afsci.145308

SANTOS, L. O., SILVA, P. G. P., LEMOS JUNIOR, W. J. F., DE OLIVEIRA, V. S., and ANSCHAU, A., 2022. Glutathione production by Saccharomyces cerevisiae: current state and perspectives. Applied Microbiology and Biotechnology, 106(5), 1879-1894. https://doi.org/10.1007/s00253-022-11826-0

SETIYONINGRUM, F., PRIADI, G., and AFIATI, F., 2022. Chemical properties of solo black garlic fermented by Saccharomyces cerevisiae. IOP Conference Series: Earth and Environmental Science, 976(1): 012044. https://doi.org/10.1088/1755-1315/976/1/012044

SITHOLE, A. N., HLATINI, V. A., and CHIMONYO, M., 2022. Potential of combining natural-derived antioxidants for improving broiler meat shelf-life - a review. Animal Bioscience, 36(9), 1305. https://doi.org/10.5713/ab.22.0188

SHAKERI, M., LE, H., SHAKERI, M., and OSKOUEIAN, E. 2020. Strategies to combat heat stress in broiler chickens: Unveiling the roles of selenium, vitamin E and vitamin C. Veterinary Sciences, 7(2), 71. https://doi.org/10.3390/vetsci7020071

SOBOTIK, E. B., NELSON, J. R., PAVLIDIS, H. O., and ARCHER, G. S., 2022. Evaluating the effects of supplementing Saccharomyces cerevisiae in the feed or drinking water on stress susceptibility of broilers. Journal of Applied Poultry Research, 31(1), 100220. https://doi.org/10.1016/j.japr.2021.100220

SUGIHARTO, S. 2016. Role of nutraceuticals in gut health and growth performance of poultry. Journal of the Saudi Society of Agricultural Sciences, 15, 99-111. http://dx.doi.org/10.1016/j.jssas.2014.06.001

SUGIHARTO, S. 2019. A review of filamentous fungi in broiler production. Annals of Agricultural Sciences, 64(1), 1-8. https://doi.org/10.1016/j.aoas.2019.05.005

SUGIHARTO, S. 2022. Dietary strategies to alleviate high-stocking-density-induced stress in broiler chickens–a comprehensive review. Archives Animal Breeding, 65(1), 21-36. https://doi.org/10.5194/aab-65-21-2022

SUGIHARTO, S., AGUSETYANINGSIH, I., WIDIASTUTI, E., WAHYUNI, H. I., YUDIARTI, T., and SARTONO, T. A., 2023. Dietary supplementation of enzymes: An approach to mitigate ammonia emission during broiler production. Iranian Journal of Applied Animal Science, 13(4), 615-625. https://journals.iau.ir/article_708962.html

SUGIHARTO, S., YUDIARTI, T., ISROLI, I., WIDIASTUTI, E., WAHYUNI, H. I., and SARTONO, T. A., 2019. Effect of formic acid, Saccharomyces cerevisiae or their combination on the growth performance and serum indices of the Indonesian indigenous crossbred chickens. Annals of Agricultural Sciences, 64(2), 206-210. https://doi.org/10.1016/j.aoas.2019.12.004

SUMANU, V. O., CHAMUNORWA, J. P., OOSTHUIZEN, M. C., and NAIDOO, V. 2022. Adverse effects of heat stress during summer on broiler chickens production and antioxidant mitigating effects. International Journal of Biometeorology, 66(12), 2379–2393. https://doi.org/10.1007/s00484-022-02372-5

SUMANU, V. O., BYARUHANGA, C., BOSMAN, A. M., OCHAI, S. O., NAIDOO, V., OOSTHUIZEN, M. C., and CHAMUNORWA, J. P., 2023. Effects of probiotic (Saccharomyces cerevisiae) and ascorbic acid on oxidative gene damage biomarker, heat shock protein 70 and interleukin 10 in broiler chickens exposed to heat stress. Animal Gene, 28, 200150. https://doi.org/10.1016/j.angen.2023.200150

SUMANU, V. O., NAIDOO, V., OOSTHUIZEN, M. C., and CHAMUNORWA, J. P., 2024. Evaluating the efficacy of probiotics and ascorbic acid as anti-stress agents against heat stress in broiler chickens. Frontiers in Veterinary Science, 11, 1482134. https://doi.org/10.3389/fvets.2024.1482134

WANG, T., CHENG, K., LI, Q., and WANG, T., 2022. Effects of yeast hydrolysate supplementation on intestinal morphology, barrier, and anti-inflammatory functions of broilers. Animal Bioscience, 35(6), 858. https://doi.org/10.5713/ab.21.0374

Wang, T., Cheng, K., Yu, C. Y., Li, Q. M., Tong, Y. C., Wang, C., and Wang, T., 2021. Effects of a yeast-derived product on growth performance, antioxidant capacity, and immune function of broilers. Poultry Science, 100(9), 101343. https://doi.org/10.1016/j.psj.2021.101343

WANG, W., LI, Z., HAN, Q., GUO, Y., ZHANG, B., and D’INCA, R., 2016. Dietary live yeast and mannan-oligosaccharide supplementation attenuate intestinal inflammation and barrier dysfunction induced by Escherichia coli in broilers. British Journal of Nutrition, 116(11), 1878-1888. https://doi.org/10.1017/S0007114516004116

WANG, X., LIU, X., LIU, S., QU, J., YE, M., WANG, J., and LI, R., 2023. Effects of anti-stress agents on the growth performance and immune function in broiler chickens with vaccination-induced stress. Avian Pathology, 52(1), 12-24. https://doi.org/10.1080/03079457.2022.2114874

WANG, Z., ZHENG, Y., LAI, Z., KONG, Z., HU, X., ZHANG, P., YANG, Y., and LI, N. 2025. Effect of Saccharomyces cerevisiae CICC 32883 fermentation on the structural features and antioxidant protection effect of Chinese yam polysaccharide. Foods, 14(4), 564. https://doi.org/10.3390/foods14040564

XU, X., LIU, A., HU, S., ARES, I., MARTÍNEZ-LARRAÑAGA, M. R., WANG, X., and MARTÍNEZ, M. A., 2021. Synthetic phenolic antioxidants: Metabolism, hazards and mechanism of action. Food Chemistry, 353, 129488. https://doi.org/10.1016/j.foodchem.2021.129488

YERRA, V. G., NEGI, G., SHARMA, S. S., and KUMAR, A., 2013. Potential therapeutic effects of the simultaneous targeting of the Nrf2 and NF-κB pathways in diabetic neuropathy. Redox Biology, 1(1), 394-397. https://doi.org/10.1016/j.redox.2013.07.005

ZDANOWSKA-SĄSIADEK, Z., LIPIŃSKA-PALKA, P., DAMAZIAK, K., MICHALCZUK, M., GRZYBEK, W., KRUZIŃSKA, B., and MARCHEWKA, J., 2019. Antioxidant effects of phytogenic herbal-vegetable mixtures additives used in chicken feed on breast meat quality. Animal Science Papers and Report, 36(4), 393-408. 

ZHAO, Y., WANG, J., FU, Q., ZHANG, H., LIANG, J., XUE, W., and ODA, H., 2022. Characterization and antioxidant activity of mannans from Saccharomyces cerevisiae with different molecular weight. Molecules, 27(14), 4439. https://doi.org/10.3390/molecules27144439